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Paternal high-fat diet altered H3K36me3 pattern of pre-implantation embryos

Published online by Cambridge University Press:  29 November 2023

Bin Meng
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China The Assisted Reproduction Center, Northwest Women’s and Children’s Hospital, Xi’an, China
Jiahui He
Affiliation:
Cancer Center, Renmin Hospital of Wuhan University, Wuhan, Hubei Province, China
Wenbin Cao
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi’an, China
Yanru Zhang
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi’an, China
Jia Qi
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi’an, China
Shiwei Luo
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China
Chong Shen
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China
Juan Zhao
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China Department of Hematology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China
Ying Xue
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China
Pengxiang Qu
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi’an, China
Enqi Liu*
Affiliation:
Laboratory Animal Center, Xi’an Jiaotong University Health Science Centre, Xi’an, Shaanxi, China Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education of China, Xi’an, China
*
Corresponding author: Enqi Liu; Email: liuenqi@xjtu.edu.cn

Summary

The global transition towards diets high in calories has contributed to 2.1 billion people becoming overweight, or obese, which damages male reproduction and harms offspring. Recently, more and more studies have shown that paternal exposure to stress closely affects the health of offspring in an intergenerational and transgenerational way. SET Domain Containing 2 (SETD2), a key epigenetic gene, is highly conserved among species, is a crucial methyltransferase for converting histone 3 lysine 36 dimethylation (H3K36me2) into histone 3 lysine 36 trimethylation (H3K36me3), and plays an important regulator in the response to stress. In this study, we compared patterns of SETD2 expression and the H3K36me3 pattern in pre-implantation embryos derived from normal or obese mice induced by high diet. The results showed that SETD2 mRNA was significantly higher in the high-fat diet (HFD) group than the control diet (CD) group at the 2-cell, 4-cell, 8-cell, and 16-cell stages, and at the morula and blastocyst stages. The relative levels of H3K36me3 in the HFD group at the 2-cell, 4-cell, 8-cell, 16-cell, morula stage, and blastocyst stage were significantly higher than in the CD group. These results indicated that dietary changes in parental generation (F0) male mice fed a HFD were traceable in SETD2/H3K36me3 in embryos, and that a paternal high-fat diet brings about adverse effects for offspring that might be related to SETD2/H3K36me3, which throws new light on the effect of paternal obesity on offspring from an epigenetic perspective.

Type
Research Article
Copyright
© The Author(s), 2023. Published by Cambridge University Press

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