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Nuclear remodelling and the developmental potential of nuclear transferred porcine oocytes under delayed-activated conditions

Published online by Cambridge University Press:  06 June 2003

Xi-Jun Yin
Affiliation:
Major of Dairy Science, Division of Applied Life Science, GyeongSang National University, Chinju, GyeongNam 660-701, Korea
Seong-Keun Cho
Affiliation:
Major of Dairy Science, Division of Applied Life Science, GyeongSang National University, Chinju, GyeongNam 660-701, Korea
Mi-Ryeung Park
Affiliation:
Major of Dairy Science, Division of Applied Life Science, GyeongSang National University, Chinju, GyeongNam 660-701, Korea
Yeo-Jeoung Im
Affiliation:
Major of Dairy Science, Division of Applied Life Science, GyeongSang National University, Chinju, GyeongNam 660-701, Korea
Joung-Ju Park
Affiliation:
Major of Dairy Science, Division of Applied Life Science, GyeongSang National University, Chinju, GyeongNam 660-701, Korea
Jong-Sik Bhak
Affiliation:
College of Veterinary Medicine, GyeongSang National University, Chinju, GyeongNam 660-701, Korea
Deug-Nam Kwon
Affiliation:
Major of Dairy Science, Division of Applied Life Science, GyeongSang National University, Chinju, GyeongNam 660-701, Korea
Sun Hong Jun
Affiliation:
Department of Animal Science, College of Agriculture, Chungbuk National University, Cheong-Ju, Chungbuk 361-763, Korea
Nam-Hyung Kim
Affiliation:
Department of Animal Science, College of Agriculture, Chungbuk National University, Cheong-Ju, Chungbuk 361-763, Korea
Jin-Hoi Kim
Affiliation:
Major of Dairy Science, Division of Applied Life Science, GyeongSang National University, Chinju, GyeongNam 660-701, Korea

Abstract

It is still unclear whether nuclear envelope breakdown and premature chromosome condensation are essential for the reprogramming of the donor nucleus following somatic nuclear transfer. To address this, we determined the ability of delayed-activated or simultaneously activated porcine oocytes to undergo nuclear remodelling and development following somatic cell nuclear transfer. A small microtubule aster was observed in association with decondensed chromatin following nuclear transfer, suggesting the introduction of a somatic cell centrosome. In the delayed-activated condition, most fibroblast nuclei divided into two chromosome masses and two pronuclear-like structures following transfer into oocytes. In contrast, fibroblast nuclei in the simultaneously activated condition formed a large, swollen, pronuclear-like structure. Microtubule asters were organised in the vicinity of the nucleus regardless of the number of nuclei. More reconstructed oocytes developed to the blastocyst stage in the delayed-activated condition than in the simultaneously activated condition (p < 0.05). Nine piglets were born from two recipient sows following transfer of delayed-activated reconstructed oocytes, while none developed to full term in the simultaneously activated condition. Fingerprint analysis showed that the PCR-RFLP patterns of the nine offspring were identical to that of the donor pig. These results suggest that the activation of recipient oocytes during nuclear transfer probably relates to the nuclear remodelling process, which can affect the ability of embryos created by somatic cell nuclear transfer to develop.

Type
Research Article
Copyright
2003 Cambridge University Press

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