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The influence of clinical and laboratory factors on the formation of monopronucleated zygotes after intracytoplasmic sperm injection (ICSI)

Published online by Cambridge University Press:  25 February 2019

Gemma Fabozzi Open the ORCID record for Gemma Fabozzi [Opens in a new window] ,
Emilia Rega ,
Maria Flavia Starita ,
Maria Giulia Amendola ,
Antonio Colicchia ,
Pierluigi Giannini  and
Claudio Piscitelli
Gemma Fabozzi*
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Emilia Rega
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Maria Flavia Starita
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Maria Giulia Amendola
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Antonio Colicchia
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Pierluigi Giannini
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
Claudio Piscitelli
Affiliation:
FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy
*
Author for correspondence: Gemma Fabozzi, FertiClinic, Casa di Cura Villa Margherita, Viale di Villa Massimo 48, 00161 Rome, Italy. Tel: +39 06 94443140. Fax: +39 06 86275838. E-mail: gemmafabozzi@hotmail.it
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Summary

The aim of the present study was to determine whether clinical or laboratory factors can influence the development of single pronucleated zygotes (1PN) and two polar bodies (PB) after ICSI. In total, 341 ICSI cycles performed at FertiClinic-Villa Margherita from January 2012 to December 2014 were enrolled in the study. Group A included 240 cycles with no 1PN−2PB while group B included 101 cycles with one or more 1PN−2PB. Age, stimulation protocol, infertility factor, amount of gonadotropin administered, duration of therapy, peak estradiol levels, number of follicles at maturation triggering, oocytes retrieved and mature oocytes, time between retrieval and injection and sperm characteristics were compared between groups. In opposition to previous results showing no relationship between 1PN occurrence and clinical or laboratory variables, we observed that 1PN−2PB zygote formation seems to be associated with a lower female age, higher level of E2 and higher number of follicles on day of oocyte maturation triggering, higher number of astenozoospermic male patients, more oocytes retrieved at pick-up, more mature oocytes (MII) and longer time to injection.

Keywords

GonadotropinsICSIMale infertilityMonopronucleated zygotesStimulation protocol.
Type
Research Article
Information
Zygote , Volume 27 , Issue 2 , April 2019 , pp. 64 - 68
Copyright
© Cambridge University Press 2019 

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