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Effect of dehydroleucodine on meiosis reinitiation in Bufo arenarum denuded oocytes

Published online by Cambridge University Press:  01 May 2007

G. Sánchez Toranzo ,
O.S. Giordano ,
L.A. López  and
M.I. Bühler
G. Sánchez Toranzo
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco, San Miguel de Tucumán, Argentina
O.S. Giordano
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco, San Miguel de Tucumán, Argentina
L.A. López
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco, San Miguel de Tucumán, Argentina
M.I. Bühler*
Affiliation:
Departamento de Biología del Desarrollo, Chacabuco, San Miguel de Tucumán, Argentina
*
All correspondence to: Marta I. Bühler, Departamento de Biología del Desarrollo, Chacabuco 461, 4000 San Miguel de Tucumán, Argentina. Fax: +54 381 4248025. e-mail: mbuhler@fbqf.unt.edu.ar
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Summary

In amphibian oocytes meiosis, the transition from G2 to M phase is regulated by the maturation promoting factor (MPF), a complex of the cyclin-dependent kinase p34/cdc2 and cyclin B. In immature oocytes there is an inactive complex (pre-MPF), in which cdc2 is phosphorylated on both Thr-161 and Thr-14/Tyr-15 residues. The dephosphorylation of Thr-14/Tyr-15 is necessary for the start of MPF activation and it is induced by the activation of cdc25 phosphatase. Late, to complete the activation, a small amount of active MPF induces an auto-amplification loop of MPF stimulation (MPF amplification). Dehydroleucodine (DhL) is a sesquiterpenic lactone that inhibits mammalian cell proliferation in G2. We asked whether DhL interferes with MPF activation. For this question, the effect of DhL (up to 30 μM) on the resumption of meiosis was evaluated, and visualized by germinal vesicle break down (GVBD), of Bufo arenarum oocytes induced in vitro by either: (i) removing follicle cells; (ii) progesterone stimulation; (iii) VG-content injection; or (iv) injection of mature cytoplasm. The results show that DhL induced GVBD inhibition, in a dose-dependent manner, in spontaneous and progesterone-induced oocyte maturation. Nevertheless, DhL at the doses assayed had no effect on GVBD induced by mature cytoplasm injection, but exerted an inhibitory effect on GVBD induced by GV content. On the basis of these results, we interpreted that DhL does not inhibit MPF amplification and that the target of DhL is any event in the early stages of the cdc25 activation cascade.

Keywords

AmphibiaDehydroleucodineMeiosis reinitiation
Type
Research Articles
Information
Zygote , Volume 15 , Issue 2 , May 2007 , pp. 183 - 187
Copyright
Copyright © Cambridge University Press 2007

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