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Effect of age, GV transfer and modified nucleocytoplasmic ratio on PKCα in mouse oocytes and early embryos

Published online by Cambridge University Press:  14 January 2011

Long-Bo Cui*
Affiliation:
Department of Biology, Yantai University, Yantai 264005, P. R. China.
Zhen-Jun Zhao
Affiliation:
Department of Biology, Yantai University, Yantai 264005, P. R. China.
Xue-Ying Zhou
Affiliation:
Department of Biology, Yantai University, Yantai 264005, P. R. China.
Qian Li
Affiliation:
Department of Biology, Yantai University, Yantai 264005, P. R. China.
Xiu-Ying Huang
Affiliation:
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
Fang-Zhen Sun
Affiliation:
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, P. R. China.
*
All correspondence to: Long-Bo Cui, Department of Biology, Yantai University, Yantai 264005, P. R. China. Tel: +86 535 6903162. e-mail: lbcui@163.com

Summary

Protein kinase C (PKC) is a family of Ser/Thr protein kinases that can be activated by Ca2+, phospholipid and diacylglycerol. There is evidence that PKC plays key roles in the meiotic maturation and activation of mammalian oocytes. The present study aimed to monitor the effect of age, germinal vesicle (GV) transfer and modified nucleoplasmic ratio on the subcellular distribution profile of PKCα, an important isozyme of PKC, in mouse oocytes undergoing meiotic maturation and following egg activation. Germinal vesicle oocytes were collected from 6–8-week-old and 12-month-old mice. Germinal vesicle-reconstructed oocytes and GV oocytes with one-half or one-third of the original oocyte volume were created using micromanipulation and electrofusion. The subcellular localization of PKCα was detected by immunocytochemistry and laser confocal microscopy. Our study showed that PKCα had a similar location pattern in oocytes and early embryos from young and old mice. PKCα was localized evenly in ooplasm, with weak staining in GV at the GV stage, and present in the entire meiosis II (MII) spindle at the MII stage. In pronuclear and 2-cell embryos, PKCα was concentrated in the nucleus except for the nucleolus. After the GV oocytes were reconstructed, the resultant MII oocytes and embryos showed a similar distribution of PKCα between reconstructed and unreconstructed controls. After one-half or two-thirds of the cytoplasm was removed from the GV oocytes, PKCα still had a similar location pattern in MII oocytes and early embryos from the GV oocytes with modified nucleoplasmic ratio. Our study showed that age, GV transfer and modified nucleocytoplasmic ratio does not affect distribution of PKCα during mouse oocyte maturation, activation, and early embryonic mitosis.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2011

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