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Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection

Published online by Cambridge University Press:  01 May 2006

Takuma Fujihira
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Mariko Kobayashi
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
Shinichi Hochi
Affiliation:
Faculty of Textile Science and Technology, Shinshu University, Ueda, Nagano 386-8567, Japan.
Masumi Hirabayashi
Affiliation:
National Institute for Physiological Sciences, Okazaki, Aichi 444-8787, Japan.
Hajime Ishikawa
Affiliation:
The Institute of Cetacean Research, Tokyo 104-0055, Japan.
Seiji Ohsumi
Affiliation:
The Institute of Cetacean Research, Tokyo 104-0055, Japan.
Yutaka Fukui*
Affiliation:
Laboratory of Animal Reproduction, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan.
*
All correspondence to: Y. Fukui. Fax: +81 155 495593. e-mail: fukui@obihiro.ac.jp

Summary

The present study investigated the effects of the sexual maturity of oocyte donors on in vitro maturation (IVM) and the parthenogenetic developmental capacity of fresh minke whale oocytes. The effects of cytochalasin B (CB) pretreatment and two types of cryoprotectant solutions (ethylene glycol (EG) or ethylene glycol and dimethylsulfoxide (EG + DMSO)) on the in vitro maturation of vitrified immature whale oocytes were compared, and the developmental capacity of vitrified immature whale oocytes following IVM and intracytoplasmic sperm injection examined (ICSI). The maturation rate did not differ significantly with sexual maturity (adult, 60.9%; prepubertal, 53.1%), but the parthenogenetic activation rate of oocytes from adult donors (76.7%) was significantly higher (p < 0.05) than that of oocytes from prepubertal donors (46.4%). The maturation rates after vitrification and warming were not significantly different between the EG (22.2%) and EG + DMSO groups (30.2%), or between the CB-treated (30.4%) and non-CB-treated groups (27.3%). These results indicate that parthenogenetic activation of in vitro matured oocytes from adult minke whales was superior to that from prepubertal whales, but that the developmental capacity of the whale oocytes after parthenogenetic activation or ICSI was still low. The present study also showed that CB treatment before vitrification and two kinds of cryoprotectants did not improve the IVM rate following the vitrification of immature whale oocytes.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2006

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References

Asada, M., Tetsuka, M., Ishikawa, H., Ohsumi, S. & Fukui, Y. (2001). Improvement on. in vitro maturation, fertilization and development of minke whale (Balaenoptera acutorostrata) oocytes. Theriogenology 56, 521–33.CrossRefGoogle ScholarPubMed
Certin, Y. & Bastan, A. (2006). Cryopreservation of immature bovine oocytes by vitrification in straws. Anim. Reprod. Sci., 92, 2936.CrossRefGoogle Scholar
Cha, K.Y., Chung, H.M., Lim, J.M., Ko, J.J., Han, S.Y., Choi, D.H. & Yoon, T.K. (2000). Freezing immature oocytes. Mol. Cell. Endocrinol. 169, 43–7.CrossRefGoogle ScholarPubMed
Chang, S.C., Jones, J.D., Ellefson, R.D. & Ryan, R.J. (1976). The porcine ovarian follicle. I. selected chemical analysis of follicular fluid at different developmental stages. Biol. Reprod. 15, 321–8.CrossRefGoogle ScholarPubMed
Dobrinsky, J.R. (2002). Advancements in cryopreservation of domestic animal embryos. Theriogenology 57, 285302.CrossRefGoogle ScholarPubMed
Dobrinsky, J.R., Pursel, V.G., Long, C.R. & Johnson, L.A. (2000). Birth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification. Biol. Reprod. 62, 564–70.CrossRefGoogle ScholarPubMed
El Mouatassim, S., Guérin, P., Ménézo, Y. (1999). Expression of genes encoding antioxidant enzymes in human and mouse oocytes during the final stages of maturation. Mol. Hum. Reprod. 5, 720–5.CrossRefGoogle ScholarPubMed
Fujihira, T., Kishida, R. & Fukui, Y. (2004). Developmental capacity of vitrified immature porcine oocytes following ICSI: effects of cytochalasin B and cryoprotectants. Cryobiology 49, 286–90.CrossRefGoogle ScholarPubMed
Fukui, Y., Mogoe, T., Terawaki, Y., Ishikawa, H., Fujise, Y. & Ohsumi, S. (1995). Relationship between physiological status and serum constituent values in minke whales (Balaenoptera acutorostrata). J. Reprod. Dev. 41, 203–8.CrossRefGoogle Scholar
Fukui, Y., Mogoe, T., Ishikawa, H. & Ohsumi, S. (1997 a). Factors affecting in vitro maturation of minke whale (Balaenoptera acutorostrata) follicular oocytes. Biol. Reprod. 56, 523–8.CrossRefGoogle ScholarPubMed
Fukui, Y., Mogoe, T., Ishikawa, H. & Ohsumi, S. (1997 b). In vitro fertilization of in vitro matured minke whale (Balaenoptera acutorostrata) follicular oocytes. Mar. Mamm. Sci. 13, 395404.CrossRefGoogle Scholar
Funahashi, H., Cantley, T.C., Stumpf, T.T., Terlouw, S.L. & Day, B.N. (1994). Use of low-salt culture medium for in vitro maturation of porcine oocytes is associated with elevated oocyte glutathione levels and enhanced male pronuclear formation after in vitro fertilization. Biol. Reprod. 51, 633–9.CrossRefGoogle Scholar
Iwata, H., Hashimoto, S., Ohota, M., Kimura, K., Shibano, K. & Miyake, M. (2004). Effects of follicle size and electrolytes and glucose in maturation medium on nuclear maturation and developmental competence of bovine oocytes. Reproduction 127, 159–64.CrossRefGoogle ScholarPubMed
Isachenko, V., Soler, C., Isachenko, E., Perez-Sanchez, F. & Grishchenko, V. (1998). Vitrification of immature porcine oocytes: effects of lipid droplets, temperature, cytoskeleton, and addition and removal of cryoprotectant. Cryobiology 36, 250–3.CrossRefGoogle ScholarPubMed
Iwayama, H., Hochi, S., Kato, M., Hirabayashi, M., Kuwayama, M., Ishikawa, H., Ohsumi, S. & Fukui, Y. (2004). Effects of cryodevice type and donors’ sexual maturity on vitrification of minke whale (Balaenoptera bonaerensis) oocytes at germinal vesicle stage. Zygote 12, 333–8.CrossRefGoogle ScholarPubMed
Iwayama, H., Ishikawa, H., Ohsumi, S. & Fukui, Y. (2005). Attempt at in vitro maturation of minke whale (Balaenoptera bonaerensis) oocytes using a portable CO2 incubator. J. Reprod. Dev. 51, 6975.CrossRefGoogle ScholarPubMed
Katayama, K.P., Stehlik, J., Kuwayama, M., Kato, O. & Stehlik, E. (2003). High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil. Steril. 80, 223–4.CrossRefGoogle ScholarPubMed
Kishida, R., Lee, E.S. & Fukui, Y. (2004). In vitro maturation of porcine oocytes using a defined medium and developmental capacity after intracytoplasmic sperm injection. Theriogenology 62, 1663–76.CrossRefGoogle ScholarPubMed
Marchal, R., Feugang, J.M., Perreau, C., Venturi, E., Terqui, M., Mermillod, P. (2001). Meiotic and developmental competence of prepubertal and adult swine oocytes. Theriogenology 56, 1729.CrossRefGoogle ScholarPubMed
O'Brien, J.K., Dwarte, D., Ryan, J.P., Maxwell, W.M.C. & Evans, G. (1996). Developmental capacity, energy metabolism and ultrastructure of mature oocytes from prepubertal and adult sheep. Reprod. Fertil. Dev. 8, 1029–37.CrossRefGoogle ScholarPubMed
Revel, F., Mermillod, P., Peynot, N., Renard, J.P. & Heyman, Y. (1995). Low developmental capacity of. in vitro matured and fertilized oocytes from calves compared with that of cows. J. Reprod. Fertil. 103, 115–20.CrossRefGoogle ScholarPubMed
Roblero, L., Biggers, J.D. & Lechene, C.P. (1976). Electron probe analysis of the elemental microenvironment of oviducal mouse embryos. J. Reprod. Fertil. 46, 431–4.CrossRefGoogle ScholarPubMed
Suzuki, M., Misumi, K., Ozawa, M., Noguchi, J., Kaneko, H., Ohnuma, K., Fuchimoto, D.I., Onishi, A., Iwamoto, M., Saito, N., Nagai, T. & Kikuchi, K. (2006). Successful piglet production by IVF of oocytes matured in vitro using NCSU-37 supplemented with fetal bovine serum. Theriogenology, 65, 374–86.CrossRefGoogle ScholarPubMed
Vieira, A.D., Mezzalira, A., Barbieri, D.P., Lehmkuhl, R.C., Rubin, M.I. & Vajta, G. (2002). Calves born after open pulled straw vitrification of immature bovine oocytes. Cryobiology 45, 91–4.CrossRefGoogle ScholarPubMed
Wang, W.H., Machaty, Z., Abeydeera, L.R., Prather, R.S. & Day, B.N. (1998). Parthenogenetic activation of pig oocytes with calcium ionophore and the block to sperm penetration after activation. Biol. Reprod. 58, 1357–66.CrossRefGoogle ScholarPubMed
Wise, T. (1987). Biochemical analysis of bovine follicular fluid: albumin, total protein, lysosomal enzymes, ions, steroids and ascorbic acid content in relation to follicular size, rank, atresia classification and day of estrous cycle. J. Anim. Sci. 64, 1153–69.CrossRefGoogle ScholarPubMed
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Developmental capacity of Antarctic minke whale (Balaenoptera bonaerensis) vitrified oocytes following in vitro maturation, and parthenogenetic activation or intracytoplasmic sperm injection
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