Hostname: page-component-848d4c4894-pjpqr Total loading time: 0 Render date: 2024-07-01T21:55:18.440Z Has data issue: false hasContentIssue false
  • English
  • Français

5-Alfa reductase type 2 (SRD5A2) gene rs523349 polymorphism is not associated with non-obstructive azoospermia in Turkish patients

Published online by Cambridge University Press:  14 October 2020

Munevver Serdarogullari Open the ORCID record for Munevver Serdarogullari [Opens in a new window] ,
Onder Coban Open the ORCID record for Onder Coban [Opens in a new window] ,
Zalihe Yarkiner ,
Seda Gulec Yilmaz ,
Tuba Akdeniz  and
Turgay Isbir
Munevver Serdarogullari*
Affiliation:
British Cyprus IVF Hospital, Embryology, Nicosia, Cyprus Cyprus International University, Faculty of Medicine, Northern Cyprus via Mersin 10, Turkey
Onder Coban
Affiliation:
British Cyprus IVF Hospital, Embryology, Nicosia, Cyprus
Zalihe Yarkiner
Affiliation:
Cyprus Science University, Software Engineering, Kyrenia, Cyprus
Seda Gulec Yilmaz
Affiliation:
Yeditepe University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey
Tuba Akdeniz
Affiliation:
Yeditepe University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey
Turgay Isbir
Affiliation:
Yeditepe University, Faculty of Medicine, Department of Medical Biology, Istanbul, Turkey
*
Author for correspondence: Munevver Serdarogullari. British Cyprus IVF Hospital, Embryology, Nicosia, Cyprus. E-mail: munevver.coban@gmail.com
Get access Rights & Permissions [Opens in a new window]

Summary

Spermatogenesis is an androgen-dependent event, and testosterone is the major androgen source. The enzyme 5-alpha reductase converts testosterone to dihydrotestosterone (DHT) in testicular and peripheral tissues. Polymorphisms in genes encoding 5-alpha reductase may be associated with impaired male fertility. The present study aimed to investigate the relationship between 5-alpha reductase type 2 (SRD5A2) gene rs523349 polymorphism and non-obstructive azoospermia (NOA) in Turkish patients. The study included 75 NOA patients and 43 fertile men from Turkey. No significant relationship was found between SRD5A2 gene rs523349 polymorphism and male infertility (P = 0.071). There was a statistically significant difference in total testosterone level and total testis volume between NOA patients and the control groups, however there was no significant difference between serum follicle-stimulating hormone and luteinizing hormone levels. Our results showed that SRD5A2 gene rs523349 polymorphism was not associated with NOA in Turkish patients.

Keywords

5-Alfa reductase type 2AzoospermiaMale infertilityNon-obstructive azoospermia
Type
Research Article
Information
Zygote , Volume 29 , Issue 2 , April 2021 , pp. 118 - 121
Copyright
© The Author(s), 2020. Published by Cambridge University Press

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Agarwal, A, Mulgund, A, Hamada, A and Chyatte, MR (2015). A unique view on male infertility around the globe. Reprod Biol Endocrinol 13, 37.CrossRefGoogle ScholarPubMed
Azzouni, F, Godoy, A, Li, Y and Mohler, J (2012). The 5 alpha-reductase isozyme family: a review of basic biology and their role in human diseases. Adv Urol 2012, 530121.CrossRefGoogle ScholarPubMed
Elzanaty, S (2013). Non-obstructive azoospermia and clinical varicocele: therapeutic options. Int Urol Nephrol 45, 669−74.CrossRefGoogle ScholarPubMed
Esteves, SC and Agarwal, A (2011). Novel concepts in male infertility. Int Braz J Urol 37, 5–15.CrossRefGoogle ScholarPubMed
Eunice, M, Philibert, P, Kulshreshtha, B, Audran, F, Paris, F, Khurana, ML, Pulikkanath, PE, Kucheria, K, Sultan, C and Ammini, AC (2008). Molecular diagnosis of 5alpha-reductase-2 gene mutation in two Indian families with male pseudohermaphroditism. Asian J Androl 10, 815–8.CrossRefGoogle ScholarPubMed
Franco, G, Scarselli, F, Casciani, V, De Nunzio, C, Dente, D, Leonardo, C, Greco, PF, Greco, A, Minasi, MG and Greco, E (2016). A novel stepwise micro-TESE approach in non obstructive azoospermia. BMC Urol 16, 20.CrossRefGoogle ScholarPubMed
Hamada, AJ, Esteves, SC and Agarwal, A (2013). A comprehensive review of genetics and genetic testing in azoospermia. Clinics (Sao Paulo) 68(Suppl 1), 39–60.CrossRefGoogle ScholarPubMed
Hines, RS, Tho, SP, Behzadian, MA and McDonough, PG (1999). Steroid 5alpha-reductase 2 gene melting polymorphisms in male subjects with azoospermia or oligospermia. Am J Obstet Gynecol 180(6 Pt 1), 1394–8.CrossRefGoogle ScholarPubMed
IBM Corp. Released (2016). IBM SPSS Statistics for Windows, Version 24.0. Armonk, NY: IBM Corp. Source: https://www-01.ibm.com/support/docview.wss?uid=swg21476197.Google Scholar
Ogino, Y, Miyagawa, S and Iguchi, T (2016). Testosterone/dihydrotestosterone. In Handbook of Hormones (eds Y. Takei, H. Ando and K. Tsutsui), pp. 515-e594E-513. San Diego: Academic Press.Google Scholar
Payne, AH, Kawano, A and Jaffe, RB (1973). Formation of dihydrotestosterone and other 5 alpha-reduced metabolites by isolated seminiferous tubules and suspension of interstitial cells in a human testis. J Clin Endocrinol Metab 37, 448–53.CrossRefGoogle Scholar
Peters, M, Saare, M, Kaart, T, Haller-Kikkatalo, K, Lend, AK, Punab, M, Metspalu, A and Salumets, A (2010). Analysis of polymorphisms in the SRD5A2 gene and semen parameters in Estonian men. J Androl 31, 372–8.CrossRefGoogle ScholarPubMed
Pezzella, A, Barbonetti, A, D’Andrea, S, Necozione, S, Micillo, A, Di Gregorio, A, Francavilla, F and Francavilla, S (2014). Ultrasonographic caput epididymis diameter is reduced in non-obstructive azoospermia compared with normozoospermia but is not predictive for successful sperm retrieval after TESE. Hum Reprod 29, 1368–74.CrossRefGoogle Scholar
Popal, W and Nagy, ZP (2013). Laboratory processing and intracytoplasmic sperm injection using epididymal and testicular spermatozoa: what can be done to improve outcomes? Clinics (Sao Paulo) 68(Suppl 1), 125–30.CrossRefGoogle ScholarPubMed
Robaire, B and Viger, RS (1995). Regulation of epididymal epithelial cell functions. Biol Reprod 52, 226–36.CrossRefGoogle ScholarPubMed
Suzuki, Y, Sasagawa, I, Itoh, K, Ashida, J and Ogata, T (2002). 5Alpha-reductase type 2 genes in Japanese males do not appear to be associated with cryptorchidism. Fertil Steril 78, 330–4.CrossRefGoogle Scholar
Walker, WH (2011). Testosterone signaling and the regulation of spermatogenesis. Spermatogenesis 1, 116–20.CrossRefGoogle ScholarPubMed
WHO (2010). World Health Organization (WHO) laboratory manual for the examination and processing of human semen.Google Scholar
Wosnitzer, M, Goldstein, M and Hardy, MP (2014). Review of azoospermia. Spermatogenesis 4, e28218.CrossRefGoogle ScholarPubMed
Zhao, D, Wu, W, Xu, B, Niu, X, Cui, H, Zhang, Y, Wang, Z and Wang, X (2012). Variants in the SRD5A2 gene are associated with quality of semen. Mol Med Rep 6, 639–44.CrossRefGoogle ScholarPubMed