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Rigid Ryegrass (Lolium rigidum) Populations Containing a Target Site Mutation in EPSPS and Reduced Glyphosate Translocation Are More Resistant to Glyphosate

Published online by Cambridge University Press:  20 January 2017

Yazid Bostamam ,
Jenna M. Malone ,
Fleur C. Dolman ,
Peter Boutsalis  and
Christopher Preston
Yazid Bostamam
Affiliation:
School and Agriculture, Food and Wine, University of Adelaide, PMB1 Glen Osmond, South Australia, 5064
Jenna M. Malone*
Affiliation:
School and Agriculture, Food and Wine, University of Adelaide, PMB1 Glen Osmond, South Australia, 5064
Fleur C. Dolman
Affiliation:
School and Agriculture, Food and Wine, University of Adelaide, PMB1 Glen Osmond, South Australia, 5064
Peter Boutsalis
Affiliation:
School and Agriculture, Food and Wine, University of Adelaide, PMB1 Glen Osmond, South Australia, 5064
Christopher Preston
Affiliation:
School and Agriculture, Food and Wine, University of Adelaide, PMB1 Glen Osmond, South Australia, 5064
*
Corresponding author's E-mail: jenna.malone@adelaide.edu.au
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Abstract

Glyphosate is widely used for weed control in the grape growing industry in southern Australia. The intensive use of glyphosate in this industry has resulted in the evolution of glyphosate resistance in rigid ryegrass. Two populations of rigid ryegrass from vineyards, SLR80 and SLR88, had 6- to 11-fold resistance to glyphosate in dose-response studies. These resistance levels were higher than two previously well-characterized glyphosate-resistant populations of rigid ryegrass (SLR77 and NLR70), containing a modified target site or reduced translocation, respectively. Populations SLR80 and SLR88 accumulated less glyphosate, 12 and 17% of absorbed glyphosate, in the shoot in the resistant populations compared with 26% in the susceptible population. In addition, a mutation within the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) where Pro106 had been substituted by either serine or threonine was identified. These two populations are more highly resistant to glyphosate as a consequence of expressing two different resistance mechanisms concurrently.

Keywords

Glyphosaterigid ryegrass, Lolium rigidum Gaud. LOLRI5-enolpyruvylshikimate-3-phosphate synthaseglyphosate absorptionherbicide resistancevineyard
Type
Weed Management
Information
Weed Science , Volume 60 , Issue 3 , September 2012 , pp. 474 - 479
Copyright
Copyright © Weed Science Society of America 

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