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Microarray Analysis of the Semicompatible, Pathogenic Response and Recovery of Leafy Spurge (Euphorbia esula) Inoculated with the Cassava Bacterial Blight Pathogen Xanthomonas axonopodis pv. manihotis

Published online by Cambridge University Press:  20 January 2017

David P. Horvath ,
María A. Santana  and
James V. Anderson
David P. Horvath*
Affiliation:
Sunflower and Plant Biology Research Unit, Red River Valley Agricultural Research Center, U.S. Department of Agriculture–Agricultural Research Service, Fargo, ND 58102
María A. Santana
Affiliation:
Departamento de Biología Celular, División de Ciencias Biológicas, Universidad Simón Bolivar, Caracas, Venezuela, and the Instituto de Estudios Avanzados, Centro de Biotecnología, Caracas, Venezuela
James V. Anderson
Affiliation:
Sunflower and Plant Biology Research Unit, Red River Valley Agricultural Research Center, U.S. Department of Agriculture–Agricultural Research Service, Fargo, ND 58102
*
Corresponding author's E-mail: David.horvath@ars.usda.gov
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Abstract

Infection by Xanthomonas axonopodis pv. manihotis (Xam) of the perennial rangeland weed leafy spurge was tested to see whether Xam might serve a potential biological control agent for this invasive weed. Although leafy spurge was susceptible to Xam infection, it recovered within 21 d after inoculation (DAI). Microarray resources available for leafy spurge allowed us to follow the physiological and signaling pathways that were altered as leafy spurge was infected and then recovered from Xam infection. The first physiological effect of Xam infection was a down-regulation of photosynthetic processes within 1 DAI. By 7 DAI, numerous processes associated with well-documented pathogenesis responses of plants were observed. Although some pathogenesis responses were still detectable at 21 DAI, other processes associated with meristem development were noted. Ontological analysis of potential signaling systems indicated jasmonic acid plays a significant role in the recovery processes.

Keywords

Leafy spurge, Euphorbia esula L. EPHESNetwork analysispathogenesistranscriptomics
Type
Weed Biology and Ecology
Information
Weed Science , Volume 61 , Issue 3 , September 2013 , pp. 428 - 436
Copyright
Copyright © Weed Science Society of America 

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