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Glyphosate-Resistant Junglerice (Echinochloa colona) from Mississippi and Tennessee: Magnitude and Resistance Mechanisms

Published online by Cambridge University Press:  11 September 2018

Vijay K. Nandula Open the ORCID record for Vijay K. Nandula [Opens in a new window] ,
Garret B. Montgomery ,
Amaranatha R. Vennapusa ,
Mithila Jugulam ,
Darci A. Giacomini ,
Jeffery D. Ray ,
Jason A. Bond ,
Lawrence E. Steckel  and
Patrick J. Tranel
Vijay K. Nandula*
Affiliation:
Research Plant Physiologist, Crop Production Systems Research Unit, USDA-ARS, Stoneville, MS, USA
Garret B. Montgomery
Affiliation:
Graduate Student, Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA
Amaranatha R. Vennapusa
Affiliation:
Postdoctoral Fellow, Department of Agronomy, Kansas State University, Manhattan, KS, USA
Mithila Jugulam
Affiliation:
Associate Professor, Department of Agronomy, Kansas State University, Manhattan, KS, USA
Darci A. Giacomini
Affiliation:
Research Assistant Professor, Department of Crop Sciences, University of Illinois, Urbana, IL, USA
Jeffery D. Ray
Affiliation:
Research Geneticist (Plants), Crop Genetics Research Unit, USDA-ARS, Stoneville, MS, USA
Jason A. Bond
Affiliation:
Professor, Delta Research and Extension Center, Mississippi State University, Stoneville, MS, USA
Lawrence E. Steckel
Affiliation:
Professor, Department of Plant Sciences, University of Tennessee, Knoxville, TN, USA
Patrick J. Tranel
Affiliation:
Professor, Department of Crop Sciences, University of Illinois, Urbana, IL, USA
*
*Author for correspondence: Vijay K. Nandula, USDA-ARS, 141 Experiment Station Road, Stoneville, MS 38776. (Email: vijay.nandula@ars.usda.gov)
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Abstract

Recently, several incidents of glyphosate failure on junglerice [Echinochloa colona (L.) Link] have been reported in the midsouthern United States, specifically in Mississippi and Tennessee. Research was conducted to measure the magnitude of glyphosate resistance and to determine the mechanism(s) of resistance to glyphosate in E. colona populations from Mississippi and Tennessee. ED50 (dose required to reduce plant growth by 50%) values for a resistant MSGR4 biotype, a resistant TNGR population, and a known susceptible MSGS population were 0.8, 1.62, and 0.23 kg ae ha−1 of glyphosate, respectively. The resistance index calculated from the these ED50 values indicated that the MSGR4 biotype and TNGR population were 4- and 7-fold, respectively, resistant to glyphosate relative to the MSGS population. The absorption patterns of [14C]glyphosate in the TNGR and MSGS populations were similar. However, the MSGS population translocated 13% more [14C]glyphosate out of the treated leaf compared with the TNGR population at 48 h after treatment. EPSPS gene sequence analyses of TNGR E. colona indicated no evidence of any point mutations, but several resistant biotypes, including MSGR4, possessed a single-nucleotide substitution of T for C at codon 106 position, resulting in a proline-to-serine substitution (CCA to TCA). Results from quantitative polymerase chain reaction analyses suggested that there was no amplification of the EPSPS gene in the resistant populations and biotypes. Thus, the mechanism of resistance in the MSGR population (and associated biotypes) is, in part, due to a target-site mutation at the 106 loci of the EPSPS gene, while reduced translocation of glyphosate was found to confer glyphosate resistance in the TNGR population.

Keywords

Prashant Jha, Montana State UniversityAbsorptionEPSPSjunglericemutationtranslocation
Type
Physiology/Chemistry/Biochemistry
Information
Weed Science , Volume 66 , Issue 5 , September 2018 , pp. 603 - 610
Copyright
© Weed Science Society of America, 2018 

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