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Metabolism of 2,4-DB by White Clover (Trifolium repens) Cell Suspension Cultures

Published online by Cambridge University Press:  12 June 2017

A. E. Smith
A. E. Smith*
Affiliation:
Dep. Agron., Univ. of Georgia, Experiment, GA 30212
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Abstract

The metabolism of 2,4-DB [4-(2,4-dichlorophenoxy)butyric acid] in cell suspension cultures of white clover (Trifolium repens L. ‘Regal Ladino’) was investigated using (ring)- 14C 2,4-DB. The herbicide and its 14C-labeled metabolites were isolated from treated cells and medium after 48 h by partition and thin layer chromatography (TLC). The major portion of the radiolabel was in the glycoside fraction (47%) and incorporated into the cell structural material (34%). The remainder of the 14C remained either as the parent herbicide (8%) or an amino acid conjugate of 2,4-DB (10%).

Keywords

Herbicide tolerancetissue cultureforagelegumes
Type
Research Article
Information
Weed Science , Volume 27 , Issue 4 , July 1979 , pp. 392 - 396
Copyright
Copyright © 1979 by the Weed Science Society of America 

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References

Literature Cited

1. Behrens, R. and Morton, H. L. 1963. Some factors influencing activity of 12 phenoxy acids on mesquite root inhibition. Plant Physiol. 38:165–170.Google Scholar
2. Carpenter, K. and Soundy, M. 1954. Investigations into the practical value of MCPB as a selective weed killer in leguminous crops. Proc. British Weed Control Conf. 1:327–345.Google Scholar
3. Clark, J. M. 1964. Experimental Biochemistry. W. H. Freeman & Co., San Francisco. 228 pp.Google Scholar
4. Feung, C. S., Hamilton, R. H., and Mumma, R. O. 1973. Metabolism of 2,4-dichlorophenoxyacetic acid. V. Identification of metabolites in soybean callus tissue cultures. J. Agric. Food Chem 21:637–640.CrossRefGoogle ScholarPubMed
5. Feung, C. S., Hamilton, R. H., and Witham, F. H. 1971. Metabolism of 2,4-dichlorophenoxyacetic acid by soybean cotyledon callus tissue cultures. J. Agric. Food Chem. 19:475–479.Google Scholar
6. Feung, C. S., Hamilton, R. H., Witham, F. H., and Mumma, R. O. 1972. The realtive amounts and identification of some 2,4-dichlorophenoxyacetic acid metabolites isolated from soybean cotyledon callus cultures. Plant Physiol. 50:80–86.CrossRefGoogle Scholar
7. Hamilton, R. H., Hunter, J., Hall, J. K., and Ercegovich, C. D. 1971. Metabolism of 2,4-dichlorophenoxy-acetic acid and 2,4,5-trichlorophenoxyacetic acid by bean plants. J. Agric. Food Chem 19:480–483.Google Scholar
8. Holley, R. W. 1952. Studies of the fate of radioactive 2,4-dichlorophenoxyacetic acid in bean plants. II. A water soluble transformation product of 2,4-D. Arch. Biochem. Biophys. 35, 171–176.Google Scholar
9. Oswald, T. H., Smith, A. E., and Phillips, D. V. 1977. Herbicide tolerance developed in cell suspension cultures of perennial white clover. Can. J. Bot. 56:1351–1358.Google Scholar
10. Sanders, J. H., Ashley, D. A., and Brown, R. H. 1977. Effects of partial defoliation on petiole phloem area, photosynthesis, and 14C translocation in developing soybean leaves. Crop Sci. 17:548–550.Google Scholar
11. Shaw, W. C., and Gentner, W. A. 1957. The selective herbicidal properties of several variously substituted phenoxyalkylcarboxylic acids. Weeds 5:75–92.Google Scholar
12. Smith, A. E. and Oswald, T. H. 1979. Degradation of phenoxyalkylcarboxylic acids by white clover cell suspensions. Weed Sci. 27:389–391.Google Scholar
13. Sunderwirth, S. G., Olson, G. G., and Johnson, G. 1964. Paper chromatography-anthrone determinations of sugars. J. Chromatogr. 16:176–180.Google Scholar
14. Synerholm, M. E. and Zimmermann, P. W. 1947. Preparation of a series of omega-(2,4-dichlorophenoxy)aliphatic acids and some related compounds with a consideration of their biochemical role as plant growth regulators. Contr. Boyce Thompson Inst. 14:369–382.Google Scholar
15. Wain, R. L. 1955. Herbicidal selectivity through specific action of plants on compounds applied. J. Agric. Food Chem. 3:128–130.Google Scholar
16. Wain, R. L. 1955. A new approach to selective weed control. Ann. Appl. Biol. 42:151–157.Google Scholar