Hostname: page-component-848d4c4894-p2v8j Total loading time: 0.001 Render date: 2024-05-30T20:34:30.225Z Has data issue: false hasContentIssue false

Factors Affecting the Presence and Persistence of Plant DNA in the Soil Environment in Corn and Soybean Rotations

Published online by Cambridge University Press:  20 January 2017

Robert H. Gulden
Affiliation:
Department of Plant Science, 222 Agriculture Building, University of Manitoba, 66 Dafoe Road, Winnipeg, MB R3T 2N2, Canada
Sylvain Lerat
Affiliation:
Department of Environmental Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
Robert E. Blackshaw
Affiliation:
Lethbridge Research Centre, Agriculture and Agri-Food Canada, 5403 1st Avenue South, Lethbridge, AB T1J 4B1, Canada
Jeff R. Powell
Affiliation:
Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
David J. Levy-Booth
Affiliation:
Department of Environmental Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
Kari E. Dunfield
Affiliation:
Department of Land Resource Science, University of Guelph, Guelph, ON N1G 2W1, Canada
Jack T. Trevors
Affiliation:
Department of Environmental Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
K. Peter Pauls
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada
John N. Klironomos
Affiliation:
Department of Integrative Biology, University of Guelph, Guelph, ON N1G 2W1, Canada
Clarence J. Swanton*
Affiliation:
Department of Plant Agriculture, University of Guelph, Guelph, ON N1G 2W1, Canada
*
Corresponding author's E-mail: cswanton@uoguelph.ca

Abstract

This study investigated factors that influence occurrence and persistence of plant DNA in the soil environment in three crop rotations. In each rotation, soil was sampled in May before planting, in July and August while crops were growing, and in October after harvest. Total DNA was recovered from soil samples taken at two different depths in the soil profile and quantified. Three target plant genes (corn CP4 epsps, corn 10-kD Zein, and soybean CP4 epsps) also were quantified in these DNA extracts using species-specific quantitative real-time PCR assays. In general, total plant DNA content in the soil environment was greatest when the crop was growing in the field and decreased rapidly after harvest. Nevertheless, low levels of target plant DNA were often still detectable the following spring. Age of rotation did not influence target DNA quantities found in the soil environment. Data were collected for a combination of 10 location-years, which allowed for estimation of the variance components for six factors including time of sampling, year, location, crop, sampling depth, and herbicide to total and target DNA content in the soil samples. Mean target recombinant DNA content in soil was influenced most strongly by time of sampling and year (85 and 6%, respectively), whereas total soil DNA content was less dynamic and was most strongly influenced by location and year (49 and 25%, respectively). Over the duration of this study, no accumulation of transgenic plant DNA in the soil environment was observed.

Type
Soil, Air, and Water
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Literature Cited

Aoshima, H., Kimura, A., Shibutani, A., Okada, C., Matsumiya, Y., and Kubo, M. 2006. Evaluation of soil bacterial biomass using environmental DNA extracted by slow-stirring method. Appl. Microbiol. Biotechnol. 71:875880.Google Scholar
Beckie, H. J., Warwick, S. I., Nair, H., and Seguin-Schwartz, G. 2003. Gene flow in commercial fields of herbicide resistant canola (Brassica napus). J. Ecol. Appl. 13:12761294.Google Scholar
Blum, S. A. E., Lorenz, M. G., and Wackernagel, W. 1997. Mechanism of retarded DNA degradation and prokaryotic origin of DNases in nonsterile soils. Syst. Appl. Microbiol. 20:513521.CrossRefGoogle Scholar
Ceccherini, M. T., Poté, J., Kay, E., Van, V. T., Marechal, J., Pietramellara, G., Nannipieri, P., Vogel, T. M., and Simonet, P. 2002. Degradation and transformability of DNA from transgenic leaves. Appl. Environ. Microbiol. 69:673683.CrossRefGoogle Scholar
Ceccherini, M. T., Poté, J., Kay, E., Van, V. T., Marechal, J., Pietramellara, G., Nannipieri, P., Vogel, T. M., and Simonet, P. 2003. Degradation and transformability of DNA from transgenic leaves. Appl. Environ. Microbiol. 69:673678.CrossRefGoogle ScholarPubMed
Crecchio, C., Ruggiero, P., Curci, M., Colombo, C., Palumbo, G., and Stotzky, G. 2005. Binding of DNA from Bacillus subtilis on montmorillonite–humic acids–aluminum or iron hydroxypolymers. Soil Sci. Soc. Am. J. 69:834841.CrossRefGoogle Scholar
Crecchio, C. and Stotzky, G. 1998. Binding of DNA on humic acids: effect on transformation of Bacillus subtilis and resistance to DNase. Soil Biol. Biochem. 30:10601067.Google Scholar
Demanèche, S., Jocteur-Monrozier, L., Quiquampoix, H., and Simonet, P. 2001. Evaluation of biological and physical protection against nuclease degradation of clay-bound plasmid DNA. Appl. Environ. Microbiol. 67:293299.Google Scholar
de Vries, J., Heine, M., Harms, K., and Wackernagel, W. 2003. Spread of recombinant DNA by roots and pollen of transgenic potato plants, identified by highly specific biomonitoring using natural transformation of an Acinetobacter sp. Appl. Environ. Microbiol. 69:44554462.CrossRefGoogle ScholarPubMed
Dubnau, D. 1999. DNA uptake in bacteria. Annu. Rev. Microbiol. 53:217244.Google Scholar
Floate, K. D., Carcamo, H. A., Blackshaw, R. E., Postman, B., and Bourassa, S. 2007. Response of ground beetle (Coleoptera: Carabidae) field populations to four years of Lepidoptera-specific Bt corn production. Environ. Entomol. 36:12691274.CrossRefGoogle ScholarPubMed
Gebhard, F. and Smalla, K. 1999. Monitoring field releases of transgenic modified sugar beets for persistence of transgenic plant DNA and horizontal gene transfer. FEMS Microbial Ecol. 28:261272.Google Scholar
Gulden, R. H., Lerat, S., Hart, M. M., Powell, J. R., Trevors, J. T., Pauls, K. P., Klironomos, J. N., and Swanton, C. J. 2005. Quantitation of transgenic plant DNA in leachate water: real-time polymerase chain reaction analysis. J. Agric. Food Chem. 53:58585865.Google Scholar
Gulden, R. H., Levy-Booth, D. J., Campbell, R., Powell, J. R., Hart, M. M., Trevors, J. T., Pauls, K. P., Klironomos, J. N., and Swanton, C. J. 2007. An empirical approach to target DNA quantification in environmental samples using real-time polymerase chain reactions. Soil Biol. Biochem. 39:19561967.Google Scholar
Hall, L. M., Topinka, A. K., Huffman, J., and Good, A. 2000. Pollen flow between herbicide-resistant Brassica napus is the cause of multiple-resistant B. napus volunteers. Weed Sci. 48:688694.Google Scholar
Hawes, M. C. 1990. Living plant cells released from the root cap: a regulator of microbial populations in the rhizosphere. Plant Soil. 129:1927.Google Scholar
Heinemann, J. A. and Traavik, T. 2004. Problems in monitoring horizontal gene transfer in field trials of transgenic plants. Nat. Biotechnol. 22:11051109.Google Scholar
Kay, E., Vogel, T. M., Bertolla, F., Nalin, R., and Simonet, P. 2002. In situ transfer of antibiotic resistance genes from transgenic (transplastomic) tobacco plants to bacteria. Appl. Environ. Microbiol. 68:33453351.Google Scholar
Lerat, S., England, L. S., Vincent, M. L., Pauls, K. P., Swanton, C. J., Klironomos, J. N., and Trevors, J. T. 2005. Real-time polymerase chain reaction (PCR) quantification of the transgenes for Roundup Ready corn and Roundup Ready soybean in soil samples. J. Agric. Food Chem. 53:13371342.CrossRefGoogle ScholarPubMed
Lerat, S., Gulden, R. H., Hart, M. M., Powell, J. R., England, L. S., Pauls, K. P., Swanton, C. J., Klironomos, J., and Trevors, J. T. 2007. Quantification and persistence of recombinant DNA of Roundup Ready crops in an agricultural soil: a field study in a corn/soybean rotation. J. Agric. Food Chem. 55:10,22610,231.Google Scholar
Levy-Booth, D. J., Campbell, R. G., Gulden, R. H., Hart, M. M., Powell, J. R., Klironomos, J. N., Pauls, K. P., Swanton, C. J., Trevors, J. T., and Dunfield, K. E. 2007. Cycling of extracellular DNA in the soil environment. Soil Biol. Biochem. 39:29772991.CrossRefGoogle Scholar
Nielsen, K. M. and Townsend, J. P. 2004. Monitoring and modeling horizontal gene transfer. Nat. Biotechnol. 22:11101114.CrossRefGoogle ScholarPubMed
Nielsen, K. M., van Elsas, J. D., and Smalla, K. 2000. Transformation of Acinetobacter sp. strain BD413(pFG4 ΔnptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants. Appl. Environ. Microbiol. 66:12371242.CrossRefGoogle ScholarPubMed
Pietramellara, G., Franchi, M., Gallori, E., and Nannipieri, P. 2001. Effect of molecular characteristics of DNA on its adsorption and binding on homoionic montmorillonite and kaolinite. Biol. Fertil. Soils. 33:402409.Google Scholar
Polverari, A., Buonaurio, R., Guiderdone, S., Pezatti, M., and Marte, M. 2000. Ultrastructural observations and DNA degradation analysis of pepper leaves undergoing a hypersensitive reaction to Xanthomonas campestris p.v. vesicatoria . Eur. J. Plant Pathol. 106:423431.Google Scholar
Poly, F., Chenu, C., Simonet, P., Rouiller, J., and Monroizer, L. F. 2000. Differences between linear chromosomal and supercoiled plasmid DNA in their mechanism and extent of adsorption to clay minerals. Langmuir. 16:12331238.CrossRefGoogle Scholar
Poté, J., Ceccherini, M. T., Van, V. T., Rosselli, W., Wildi, W., Simonet, P., and Vogel, T. M. 2003. Fate and transport of antibiotic resistance genes in saturated soil columns. Eur. J. Soil Biol. 39:6571.Google Scholar
Poté, J., Rossé, P., Rosselli, W., Van, V. T., and Wildi, W. 2005. Kinetics of mass and DNA decomposition in tomato leaves. Chemosphere. 61:677684.Google Scholar
Poté, J., Rosselli, W., Wigger, A., and Wildi, W. 2007. Release and leaching of plant DNA in unsaturated soil column. Ecotoxicol. Environ. Saf. 68:293298.Google Scholar
Reagon, M. and Snow, A. A. 2006. Cultivated Helianthus annuus (Asteraceae) volunteers as a genetic “bridge” to weedy sunflower populations in North America. Am. J. Bot. 93:127133.Google Scholar
Reanney, D. C., Roberts, W. P., and Kelly, W. J. 1982. Genetic interactions among microbial communities. Pages 287322. in Bull, A. T. S. and Slater, J. H. Microbial Interactions and Communities. London Academic Press.Google Scholar
Romanowski, G., Lorenz, M. G., and Wackernagel, W. 1991. Adsorption of plasmid DNA to mineral surfaces and protection against DNase I. Appl. Environ. Microbiol. 57:10571061.CrossRefGoogle ScholarPubMed
Swanton, C. J., Sikkema, P., Hamill, A., Tardif, F., and Gulden, R. H. 2007. Long-term effects of Roundup-ReadyTM compared to conventional herbicide systems in Ontario. http://www.plant.uoguelph.ca/research/weedsci/pdf/Monsanto_Rotation_Final_Reportv2.pdf. Accessed July 7, 2008.Google Scholar
Uribelarrea, M., Carcova, J., Otegui, M. E., and Westgate, M. E. 2002. Pollen production, pollination dynamics, and kernel set in maize. Crop Sci. 42:19101918.Google Scholar
Westgate, M. E., Lizaso, J., and Batchelor, W. 2003. Quantitative relationships between pollen shed density and grain yield in maize. Crop Sci. 43:934942.CrossRefGoogle Scholar
Widmer, F., Seidler, R. J., Donegan, K. K., and Reed, G. L. 1997. Quantification of transgenic plant marker gene persistence in the field. Mol. Ecol. 6:17.CrossRefGoogle Scholar
Wilson, I. G. 1997. Inhibition and facilitation of nucleic acid amplification. Appl. Environ. Microbiol. 63:37413751.CrossRefGoogle ScholarPubMed