Skip to main content Accessibility help
×
Home
Hostname: page-component-cf9d5c678-r9vz2 Total loading time: 0.259 Render date: 2021-07-27T06:31:01.916Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }

Article contents

Cloning and Characterization of a Critical Meristem Developmental Gene (EeSTM) from Leafy Spurge (Euphorbia esula)

Published online by Cambridge University Press:  20 January 2017

Vijaya Varanasi
Affiliation:
Department of Plant Sciences, North Dakota State University, State University Station, Fargo ND 58105
Tracey Slotta
Affiliation:
U.S. Department of Agriculture, Agricultural Research Service, Biosciences Research Laboratory, P.O. Box 5674, State University Station, Fargo, ND 58105-5674
David Horvath
Affiliation:
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
Corresponding

Abstract

SHOOTMERISTEMLESS (STM) encodes a member of the class I KNOX homeodomain protein family that is required for meristem development and maintenance. We have isolated both genomic and cDNA clones of STM from the perennial weed leafy spurge. A comparison to other class I KNOX genes indicates that EeSTM represents an orthologue of AtSTM and not one of the other class I KNOX gene family members. 5′ rapid amplification of cDNA ends (RACE) indicated that the transcription initiation site is close to the start of translation and is conserved between arabidopsis and leafy spurge. Putative cis-acting elements were identified in the EeSTM promoter, including a tuber-specific sucrose-responsive element, which could play a major role in the expression of EeSTM in root tissue.

Type
Physiology, Chemistry, and Biochemistry
Copyright
Copyright © Weed Science Society of America 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Adai, A., Johnson, C., Mlotshwa, S., Archer-Evans, S., Manocha, V., Vance, V., and Sundaresan, V. 2005. miRNA candidates in Arabidopsis . Genome Res. 15:7891. Computational prediction of miRNAs in Arabidopsis thaliana. sundarlab.ucdavis.edu/mirna/search_candidates.html. Accessed: April 17, 2008.CrossRefGoogle Scholar
Anderson, J. V., Gesch, R. W., Jia, Y., Chao, W. S., and Horvath, D. P. 2005. Seasonal shifts in dormancy status, carbohydrate metabolism, and related gene expression in crown buds of leafy spurge. Plant Cell and Env. 28:15671578.CrossRefGoogle Scholar
Anderson, J. V., Horvath, D. P., Chao, W. S., Foley, M., Hernandez, A., Thimmapuram, J., Liu, L., Gong, G., Band, M., Kim, R., and Mikel, M. 2007. Characterization of an EST database for the perennial weed leafy spurge: an important resource for weed biology research. Weed Sci. 55:193203.CrossRefGoogle Scholar
Barton, M. K. and Poethig, R. S. 1993. Formation of the shoot apical meristem in Arabidopsis thaliana: an analysis of development in the wild type and in the shoot meristemless mutant. Development. 119:823831.Google Scholar
Bowman, J. L. and Eshed, Y. 2000. Formation and maintenance of the shoot apical meristem. Trends Plant Sci. 5:110115.CrossRefGoogle ScholarPubMed
Byrne, M. E., Simorowski, J., and Martienssen, R. A. 2002. ASYMMETRIC LEAVES1 reveals knox gene redundancy in Arabidopsis . Development. 129:19571965.Google ScholarPubMed
Chao, W. S., Horvath, D. P., Anderson, J. V., and Foley, M. E. 2005. Potential model weeds to study genomics, ecology, and physiology in the 21st century. Weed Sci. 53:929937.CrossRefGoogle Scholar
Chao, W. S., Serpe, M. D., Anderson, J. V., Gesch, R. W., and Horvath, D. P. 2006. Sugars, hormones, and environment affect the dormancy status in underground adventitious buds of leafy spurge (Euphorbia esula). Weed Sci. 54:5968.CrossRefGoogle Scholar
Corpet, F. 1988. Multiple sequence alignment with hierarchical clustering. Nucl. Acids Res. 16:1088110890.CrossRefGoogle ScholarPubMed
Grbic, V. and Bleecker, A. B. 2000. Axillary meristem development in Arabidopsis thaliana . Plant J. 21:215223.CrossRefGoogle ScholarPubMed
Groover, A. T., Mansfield, S. D., DiFazio, S. P., Dupper, G., Fontana, J. R., Millar, R., and Wang, Y. 2006. The Populus homeobox gene ARBORKNOX1 reveals overlapping mechanisms regulating the shoot apical meristem and the vascular cambium. Plant Mol. Biol. 61:917932.CrossRefGoogle ScholarPubMed
Guillet-Claude, C., Isabel, N., Pelgas, B., and Bousquet, J. 2004. The evolutionary implications of KNOX-I gene duplications in conifers: correlated evidence from phylogeny, gene mapping, and analysis of functional divergence. Mol. Bio. Evol. 21:22322245.CrossRefGoogle ScholarPubMed
Higo, K., Ugawa, Y., Iwamoto, M., and Korenaga, T. 1999. Plant cis-acting regulatory DNA elements (PLACE) database: 1999. Nucleic Acids Res. 27:297300. www.dna.affrc.go.jp/PLACE/. Accessed: April 17, 2008.CrossRefGoogle ScholarPubMed
Horvath, D. P., Chao, W. S., and Anderson, J. V. 2002. Molecular analysis of signals controlling dormancy and growth in underground adventitious buds of leafy spurge. Plant Physiol. 128:14391446.CrossRefGoogle ScholarPubMed
Kidner, C. A. and Martienssen, R. A. 2005. The role of ARGONAITE (AGO1) in meristem formation and identity. Dev. Biol. 280:504517.CrossRefGoogle ScholarPubMed
Lang, G. A., Early, J. D., Darnell, R. L., and Martin, G. C. 1987. Endo-, para-, and ecodormancy: physiological terminology and classification for dormancy research. HortScience. 22:371377.Google Scholar
Le Gourrierec, J., Li, Y. F., and Zhou, D. X. 1999. Transcriptional activation by Arabidopsis GT-1 may be through interaction with TFIIA-TBP-TATA complex. Plant J. 18:663668.CrossRefGoogle ScholarPubMed
Long, J. A., Moan, E. I., Medford, J. I., and Barton, M. K. 1996. A member of the KNOTTED class of homeodomain proteins encoded by the STM gene of Arabidopsis . Nature. 379:6669.CrossRefGoogle ScholarPubMed
Murray, M. G. and Thompson, W. F. 1980. Rapid isolation of high molecular-weight plant DNA. Nucl. Acids Res. 8:4321.CrossRefGoogle ScholarPubMed
Raju, M. V. S. 1975. Experimental studies on leafy spurge (Euphorbia esula L.). I. Ontogeny and distribution of buds and shoots on the hypocotyl. Bot. Gaz. 136:254261.CrossRefGoogle Scholar
Reiser, L., Sánchez-Baracaldo, P., and Hake, S. 2000. Knots in the family tree: evolutionary relationships and functions of KNOX homeobox genes. Plant Mol. Biol. 42:151166.CrossRefGoogle ScholarPubMed
Sambrook, E., Fritsch, F., and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. 2nd ed. Cold Spring Harbor, NY Cold Spring Harbor Laboratory Press.Google Scholar
Schultz-Schaeffer, J. and Gerhardt, S. 1987. Cytotaxonomic analysis of Euphorbia ssp. (“Leafy Spurge”) complex. Biol. Zentralbl. 106:429438.Google Scholar
Scofield, S. and Murray, J. A. H. 2006. KNOX gene function in plant stem cell niches. Plant Mol. Biol. 60:929946.CrossRefGoogle ScholarPubMed
Swofford, D. L. 2000. PAUP: phylogenetic analysis using parsimony, version 4.0. Champaign, IL Illinois Natural History Survey.Google Scholar
Tsiantis, M., Schneeberger, R., Golz, J. F., Freeling, M., and Langdale, J. A. 1999. The maize rough sheath2 gene and leaf development programs in monocot and dicot plants. Science. 284:154156.CrossRefGoogle ScholarPubMed
Uchida, N., Townsley, B., Chung, K-H., and Sinha, N. 2007. Regulation of SHOOT MERISTEMLESS genes via an upstream-conserved noncoding sequence coordinates leaf development. Proc. Nat. Acad. of Sci. 104:1595315958.CrossRefGoogle ScholarPubMed
Xu, L., Xu, Y., Dong, A., Sun, Y., Pi, L., Xu, Y., and Huang, H. 2003. Novel as1 and as2 defects in leaf adaxial–abaxial polarity reveal the requirement for ASYMMETRIC LEAVES1 and 2 and ERECTA functions in specifying leaf adaxial identity. Development. 130:40974107.CrossRefGoogle ScholarPubMed
Zhu, Y. Y., Machleder, E. M., Chenchik, A., Li, R., and Siebert, P. D. 2001. Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques. 30:892897.Google Scholar
Zourelidou, M., Zabala, M., Smith, C., and Bevan, M. W. 2002. Storekeeper defines a new class of plant-specific DNA-binding proteins and is a putative regulator of patatin expression. Plant J. 30:489497.CrossRefGoogle ScholarPubMed
1
Cited by

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

Cloning and Characterization of a Critical Meristem Developmental Gene (EeSTM) from Leafy Spurge (Euphorbia esula)
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

Cloning and Characterization of a Critical Meristem Developmental Gene (EeSTM) from Leafy Spurge (Euphorbia esula)
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

Cloning and Characterization of a Critical Meristem Developmental Gene (EeSTM) from Leafy Spurge (Euphorbia esula)
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *