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Spectral sensitivity of primate photoreceptors

Published online by Cambridge University Press:  02 June 2009

J.L. Schnapf ,
T.W. Kraft ,
B.J. Nunn  and
Baylor D.A.
J.L. Schnapf
Affiliation:
Departments of Ophthalmology and Physiology, University of California, San Francisco
T.W. Kraft
Affiliation:
Department of Neurobiology, Stanford Medical School, Stanford
B.J. Nunn
Affiliation:
Department of Neurobiology, Stanford Medical School, Stanford
Baylor D.A.
Affiliation:
Department of Neurobiology, Stanford Medical School, Stanford
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Abstract

The spectral sensitivities of rods and cones in macaque and human retinas were determined by recording the membrane current from single outer segments. In the macaque retina, the wavelengths of maximum sensitivity were at about 430, 530, and 561 nm for the blue, green, and red cones, respectively, and at 491 nm for the rods. The shapes of the spectra of the three cones were similar when plotted on a log wavenumber scale; the rod spectrum was slightly broader. Spectral sensitivities of the red and green cones from a human retina were virtually identical to those of macaque cones. For comparison with human psychophysical measurements, the rod and cone spectra were adjusted to give the sensitivities expected for light incident on the cornea of the human eye. These functions satisfactorily predicted the scotopic and photopic luminosity functions as well as results from human color-matching experiments. The adjusted spectra of the red and green cones also agreed well with the π-mechanisms of Stiles (1953, 1959).

Keywords

PhotoreceptorsConesRodsSpectral sensitivityPrimateRetina
Type
Research Article
Information
Visual Neuroscience , Volume 1 , Issue 3 , May 1988 , pp. 255 - 261
Copyright
Copyright © Cambridge University Press 1988

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References

Baylor, D.A., Nunn, B.J. & Schnapf, J.L. (1984). The photocurrent, noise, and spectral sensitivity of rods of the monkey Macaca fascicularis. Journal of Physiology 357, 575–607.CrossRefGoogle ScholarPubMed
Baylor, D.A., Nunn, B.J. & Schnapf, J.L. (1987). Spectral sensitivity of cones of the monkey Macaca fascicularis. Journal of Physiology 390, 145–160.CrossRefGoogle ScholarPubMed
Bowmaker, J.K., Dartnall, H.J.A. & Mollon, J.D. (1980). Microspectrophotometric demonstration of four classes of photoreceptor in an Old World primate, Macaca fascicularis. Journal of Physiology 298, 131–143.CrossRefGoogle Scholar
Cornwall, M.C., Macnichol, E.F. & Fein, A. (1984). Absorptance and spectral sensitivity measurements of rod photoreceptors of the tiger salamander, Ambystoma tigrinum. Vision Research 24, 1651–1659.CrossRefGoogle ScholarPubMed
Crawford, B.H. (1949). The scotopic visibility function. Proceedings of the Physical Society B 62, 321–334.CrossRefGoogle Scholar
Dartnall, H.J.A., Bowmaker, J.K. & Mollon, J.D. (1983). Human visual pigments: microspectrophotometric results from the eyes of seven persons. Proceedings of the Royal Society B 220, 115–130.Google ScholarPubMed
DeValois, R., Morgan, H.C., Polson, M., Mead, W.R. & Hull, E.M. (1974). Psychophysical studies on monkey vision, I: Macaque luminosity and color vision tests. Vision Research 14, 53–67.CrossRefGoogle Scholar
Estevez, O. (1979). On the fundamental data base of normal and dichromatic color vision. Ph.D. Thesis, University of Amsterdam, Krips Repros Meppel, Amsterdam.Google Scholar
Harosi, F.I. (1975). Absorption spectra and linear dichroism of some amphibian photoreceptors. Journal of General Physiology 66, 357–382.CrossRefGoogle ScholarPubMed
Kraft, T.W. (1988). Transduction by cones in the golden-mantled ground squirrel, Citellus lateralis. Biophysical Journal 53(2), 474a.Google Scholar
Lamb, T.D., Macnaughton, P.A. & Yau, K.-Y. (1981). Spatial spread of activation and background desensitization in toad rod outer segments. Journal of Physiology 319, 463–496.CrossRefGoogle ScholarPubMed
Levine, J.S. & Macnichol, E.F. (1985). Microspectrophotometry of primate photoreceptors: Art, artifact and analysis. In The Visual System, ed. Fein, A. & Levine, J., pp 73–87. New York: A.R. Liss.Google Scholar
MacNichol, E.F., Levine, J.S., Mansfield, R.J.W., lipetz, L.E. & Collins, B.A. (1983). Microspectrophotometry of visual pigments in primate photoreceptors. In Colour Vision, ed. Mollon, J.D. & Sharpe, L.T., pp 13–38. London: Academic Press.Google Scholar
Mansfield, R.J.W. (1985). Primate photoreceptors and cone mechanisms. In The Visual System, ed. Fein, A. & Levine, J.S., pp 89–106. New York: A.R. Liss.Google Scholar
Mansfield, R.J.W., Levine, J.S., Lipetz, L.E., Collins, B.A., Raymond, G. & Macnichol, E.F. (1984). Blue sensitive cones in the primate retina: Microspectrophotometry of the visual pigment. Brain Research 56, 389–394.CrossRefGoogle ScholarPubMed
Naka, K.I. & Rushton, W.A.H. (1966). Spotentials from colour units in the retina of fish (Cyprinidae). Journal of Physiology 185, 536–555.CrossRefGoogle ScholarPubMed
Neitz, J. & Jacobs, G.H. (1986). Polymorphism of the long-wave length cone in normal human colour vision. Nature 323, 623–625.CrossRefGoogle Scholar
Schnapf, J.L., Kraft, T.W. & Baylor, D.A. (1987). Spectral sensitivity of human cone photoreceptors. Nature 325, 439–441.CrossRefGoogle ScholarPubMed
Smith, V.C. & Pokorny, J. (1972). Spectral sensitivity of color-blind observers and the cone photopigments. Vision Research 12, 2059–2071.CrossRefGoogle ScholarPubMed
Stiles, W.S. (1953). Further studies of visual mechanisms of the two-colour threshold technique. Coloquio sobre problems opticos de la vision, Madrid, Union Internationale de Physique Pure et Appliquée 1, 65–103.Google Scholar
Stiles, W.S. (1959). Color vision: the approach through increment threshold sensitivity. Proceedings of the National Academy of Sciences (USA) 45, 100–114.CrossRefGoogle Scholar
Stiles, W.S. & Burch, J.M. (1955). Interim report to the Commission Internationale de I'Eclairage, Zurich, 1955, on the National Physical Laboratory's investigation of colour-matching. Optica Acta 2, 168–181.CrossRefGoogle Scholar
Vos, J.J. (1978). Colorimetric and photometric properties of a 2° fundamental observer. Color Research and Application 3, 125–128.CrossRefGoogle Scholar
Wyszecki, G. & Stiles, W.S. (1982). Color Science. Concepts and Methods, Quantitative Data and Formulae. New York: Wiley.Google Scholar