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The rod bipolar cell of the mammalian retina

Published online by Cambridge University Press:  02 June 2009

Heinz Wässle ,
Masayuki Yamashita ,
Ursula Greferath ,
Ulrike Grünert  and
Frank Müller
Heinz Wässle
Affiliation:
Max-Planck Institut für Hirnforschung, Deutschordenstrasse 46, Frankfurt 71, Germany
Masayuki Yamashita
Affiliation:
Max-Planck Institut für Hirnforschung, Deutschordenstrasse 46, Frankfurt 71, Germany
Ursula Greferath
Affiliation:
Max-Planck Institut für Hirnforschung, Deutschordenstrasse 46, Frankfurt 71, Germany
Ulrike Grünert
Affiliation:
Max-Planck Institut für Hirnforschung, Deutschordenstrasse 46, Frankfurt 71, Germany
Frank Müller
Affiliation:
Max-Planck Institut für Hirnforschung, Deutschordenstrasse 46, Frankfurt 71, Germany
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Abstract

Three approaches to study the function of mammalian rod bipolar cells are described. Extracellular recordings from the intact cat eye under light- and dark-adapted conditions showed that in dark-adapted retina all light responses can be blocked by 2-amino-4-phosphonobutyrate (APB). Immunocytochemical staining with an antibody against protein kinase C (PKC) labeled rod bipolar cells in all mammalian retinae tested. When rat retinae were dissociated, PKC immunoreactivity was also found in isolated bipolar cells and could be used for their identification as rod bipolars. Patch-clamp recordings were performed from such dissociated rod bipolar cells and their responses to APB were measured. APB closed a nonselective cation channel in the cell membrane. The actions of GABA and glycine were also tested and both opened chloride channels in dissociated rod bipolar cells. These results suggest that rod bipolar cells are depolarized by a light stimulus and that GABA as well as glycine modulate their light responses.

Keywords

Rod bipolar cells2-amino-4-phosphonobutyrate (APB)patch-clamp recordingsprotein kinase C immunocytochemistry
Type
Research Articles
Information
Visual Neuroscience , Volume 7 , Issue 1-2 , August 1991 , pp. 99 - 112
Copyright
Copyright © Cambridge University Press 1991

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