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Multiple subtypes of glycine-immunoreactive neurons in the goldfish retina: Single- and double-label studies

Published online by Cambridge University Press:  02 June 2009

Stephen Yazulla  and
Keith M. Studholme
Stephen Yazulla
Affiliation:
Department of Neurobiology and Behavior, State University of New York, Stony Brook
Keith M. Studholme
Affiliation:
Department of Neurobiology and Behavior, State University of New York, Stony Brook
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Abstract

The glycinergic system in goldfish retina was studied by immunocytochemical localization of glycine antiserum at the light-microscopical level. Numerous amacrine cells, a type of interplexiform cell, interstitial cell, and displaced amacrine cell were glycine-immunoreactive (IR). Amacrine cells, accounting for 97% of the glycine-IR neurons, were of four types based solely on their level of dendritic stratification: stratified amacrine cells of the first, third, and fifth sublayers and bistratified amacrine cells of the first and fifth sublayers. Double-labeling experiments were carried out to determine possible co-localization of glycine-IR with GABA-IR, serotonin-IR, substance P-IR and somatostatin-IR. No evidence for co-localization of glycine-IR with these other transmitter substances was found, despite reports of co-localization of these substances in retinas of other species. Glycinergic neurons in goldfish retina appear to consist of a heterogeneous population of at least seven morphologically distinct subtypes that are also neurochemically distinct in regard to GABA, serotonin, substance P, and somatostatin. Since dendritic stratification in the inner plexiform layer is correlated with ON-, OFF-response types, we suggest that the subtypes of glycine-IR amacrine cells play different roles in the encoding of visual information.

Keywords

RetinaGoldfishAmacrine cellsGlycineImmunocytochemistryColocalizationNeuotransmitters
Type
Research Articles
Information
Visual Neuroscience , Volume 4 , Issue 3 , March 1990 , pp. 299 - 309
Copyright
Copyright © Cambridge University Press 1990

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