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Identification and localization of K+ channels in the mouse retina

Published online by Cambridge University Press:  02 June 2009

David J. Klumpp ,
Eun Joo Song  and
Lawrence H. Pinto
David J. Klumpp
Affiliation:
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston
Eun Joo Song
Affiliation:
Department of Neurobiology and Physiology, Northwestern University, Evanston
Lawrence H. Pinto
Affiliation:
Department of Neurobiology and Physiology, Northwestern University, Evanston
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Abstract

Voltage-gated potassium channels are differentially expressed in the brain, and recent studies have shown that K+ channels show subcellular localization. We characterized the distribution of five different K+ channels in the mouse retina. Each channel was distributed in a unique pattern in the retina and was localized to specific subcellular domains within a given retinal neuron. Kvl.4 and Kv4.2 were consistently found in axonal and somatodendritic portions, respectively, consistent with previous studies in brain. In contrast, Kvl.2, Kvl.3, and Kv2.1 showed variable subcellular distribution depending upon cellular context. These results suggest that no one K+ channel is distributed over the entire length of the neuron to provide a “housekeeping” level of membrane potential stabilization. Instead, we propose that each K+ channel is associated with a specific subcellular functional module, and each local K+ conductance responds uniquely to local voltage and second messenger signals.

Keywords

K+ channelRetinaLocalizationMouseRT PCR
Type
Research Articles
Information
Visual Neuroscience , Volume 12 , Issue 6 , November 1995 , pp. 1177 - 1190
Copyright
Copyright © Cambridge University Press 1995

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