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Cell type-specific and light-dependent expression of Rab1 and Rab6 GTPases in mammalian retinas

Published online by Cambridge University Press:  11 December 2009

WEI HUANG ,
GUANGYU WU  and
GUO-YONG WANG
WEI HUANG
Affiliation:
Department of Structural and Cellular Biology, School of Medicine, Tulane University, New Orleans, Louisiana
GUANGYU WU
Affiliation:
Department of Pharmacology, Louisiana State University Health Sciences Center, New Orleans, Louisiana
GUO-YONG WANG*
Affiliation:
Department of Structural and Cellular Biology, School of Medicine, Tulane University, New Orleans, Louisiana
*
*Address correspondence and reprint requests to: Guo-Yong Wang, Department of Structural and Cellular Biology, School of Medicine, Tulane University, 1430 Tulane Avenue, SL-49, New Orleans, LA 70112. E-mail: gwang@tulane.edu
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Abstract

The Ras-like Rab1 and Rab6 GTPases modulate protein traffic along the early secretory pathway and are involved in the regulation of maturation of rhodopsin in the outer retina. However, Rab GTPases have not been studied in the inner retinas. Here, we analyzed the anatomatic distribution and expression of Rab1 and Rab6 in the mouse and rat retinas by immunohistochemistry and immunoblotting. We found that Rab1 was specifically expressed in the rod bipolar cells, while Rab6 was expressed in a different cell type(s) from rod bipolar cells in the inner retina. We also demonstrated that expression of Rab1 and Rab6 was increased with light. These data provided the first evidence implicating that Rab1 and Rab6 may be involved in the regulation of the retinal adaptation.

Keywords

Protein kinase CMüller CellInner nuclear layerRod bipolar cellRetinal adaptation
Type
Research Articles
Information
Visual Neuroscience , Volume 26 , Issue 5-6 , November 2009 , pp. 443 - 452
Copyright
Copyright © Cambridge University Press 2009

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