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Localization of actin filaments and microtubules in the cells of the Limulus lateral and ventral eyes

Published online by Cambridge University Press:  02 June 2009

Bruce G. Calman  and
Steven C. Chamberlain
Bruce G. Calman
Affiliation:
Institute for Sensory Research, Syracuse University, Syracuse
Steven C. Chamberlain
Affiliation:
Institute for Sensory Research, Syracuse University, Syracuse Department of Bioengineering, Syracuse University, Syracuse
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Abstract

The ommatidia of the lateral eye of the horseshoe crab, Limulus polyphemus, undergo rhythmic changes in structure that are driven by diurnal lighting and efferent neural activity from a circadian clock in the brain. This study uses cytochemical probes to investigate the cytoskeletal elements mediating these responses and to develop models for their control. Antibodies to actin and phalloidin, a specific F-actin probe, label the rhabdom of lateral eye ommatidia, the cone cells of the ommatidial aperture, the ommatidial sheath, and the peripheral regions of the photoreceptor (retinular cell) cytoplasm. These probes also label the rhabdomere of ventral photoreceptors. Antibodies to tubulin label the eccentric cell dendrite and soma in each lateral eye ommatidium, the cone cells of the aperture, and the peripheral retinular cell cytoplasm. Models are proposed for the cytoskeletal mechanisms involved in controlling aperture and rhabdom shape, pigment movement, and shedding of rhabdomeral membrane.

Keywords

CytoskeletonLimulusImmunocytochemistryPhotoreceptorsRetina
Type
Research Articles
Information
Visual Neuroscience , Volume 9 , Issue 3-4 , October 1992 , pp. 365 - 375
Copyright
Copyright © Cambridge University Press 1992

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