GWG, which is the weight gained by the mother during pregnancy, is a complex trait in which both fetal environmental (e.g., intrauterine exposure to maternal diabetes), maternal environmental (e.g., diet and physical activity during pregnancy), and physiological factors are involved (Dabelea et al., Reference Dabelea, Hanson, Lindsay, Pettitt, Imperatore, Gabir and Knowler2000; Lawlor et al., Reference Lawlor, Fraser, Macdonald-Wallis, Nelson, Palmer, Davey Smith and Tilling2011; Ludwig & Currie, Reference Ludwig and Currie2010). Numerous studies have shown that variation in GWG can predict a wide variety of short- and long-term health outcomes in both mother (Fraser et al., Reference Fraser, Tilling, Macdonald-Wallis, Hughes, Sattar, Nelson and Lawlor2011; Gaillard et al., Reference Gaillard, Durmus, Hofman, Mackenbach, Steegers and Jaddoe2013; Mannan et al., Reference Mannan, Doi and Mamun2013) and offspring (Beyerlein et al., Reference Beyerlein, Nehring, Rzehak, Heinrich, Mluler, Plachta-Danielzik and von Kries2012; Ludwig et al., Reference Ludwig, Rouse and Currie2013; Reynolds et al., Reference Reynolds, Osmond, Phillips and Godfrey2010; Schack-Nielsen et al., Reference Schack-Nielsen, Michaelsen, Gamborg, Mortensen and Sorensen2010), such as gestational hypertension, cesarean delivery, post-partum weight retention, and maternal and offspring overweight and obesity (Gaillard et al., Reference Gaillard, Durmus, Hofman, Mackenbach, Steegers and Jaddoe2013; Mannan et al., Reference Mannan, Doi and Mamun2013; Reynolds et al., Reference Reynolds, Osmond, Phillips and Godfrey2010; Schack-Nielsen et al., Reference Schack-Nielsen, Michaelsen, Gamborg, Mortensen and Sorensen2010). However, the extent to which environmental and genetic factors contribute to the phenotypic variation in GWG is unknown. There is also evidence that the variation of a specific trait explained by genetic factors can vary over time, concurrent with change in environmental factors (Rokholm et al., Reference Rokholm, Silventoinen, Tynelius, Gamborg, Sorensen and Rasmussen2011; Silventoinen et al., Reference Silventoinen, Pietilainen, Tynelius, Sorensen, Kaprio and Rasmussen2007). Whether the influences of genetic factors that explain the variation in GWG change over time (e.g., from one pregnancy to the next) remains unknown.
A common method to investigate the genetic and environmental sources of variation is to utilize twin design where the resemblance of MZ and DZ twin pairs is compared (Rijsdijk & Sham, Reference Rijsdijk and Sham2002). The heritability of a specific trait is the proportion of variability of the phenotype that can be attributed to genetic variation (Boomsma et al., Reference Boomsma, Busjahn and Peltonen2002). Twin study design allows decomposition of the phenotypic variance into additive and dominance genetic, common environmental (factors shared by co-twins), and unique environmental factors (specific to each twin sister; Kaprio & Silventoinen, Reference Kaprio and Silventoinen2011); the latter component also includes any residual variation.
Knowledge from classical twin research on genetic and environmental influences on GWG may guide further research exploring specific genetic and environmental factors and their possible interactions, with the possibility of becoming future targets for preventing excessive GWG. We are not aware of previous studies that have investigated the heritability of GWG. Thus, our main objective was to explore the extent to which GWG can be explained by genetic, shared environmental, and/or unique environmental factors in the first pregnancy. To see whether the variation in GWG explained by genetic factors differs according to pregnancy order, we also analyzed the heritability of GWG in the mothers’ second pregnancy. Our secondary objective was to investigate whether different sets of genes and/or environmental factors influence the variation in GWG in two consecutive pregnancies.
Materials and Methods
Register Linkage and Subjects
Our population-based twin cohort study was created through the linkage of the Medical Birth Register (MBR; Axelsson, Reference Axelsson2003), the Swedish Twin Register (STR; Lichtenstein et al., Reference Lichtenstein, Sullivan, Cnattingius, Gatz, Johansson, Carlstrom and Pedersen2006), and the Multi-Generation Register (MGR; Ekbom, Reference Ekbom2011), by using the unique personal identification number assigned to all Swedish residents. The target population consisted of all Swedish female twins born 1945–1986 who had given birth to at least one singleton child for the main univariate analyses, or two consecutive singleton children for the subanalysis, during 1982–1989 and 1992–2010 (no data was available on GWG during 1990–1991 in the MBR). The univariate analyses were carried out in both the mothers’ first and second pregnancies (separately), and for the bivariate analysis, the mothers’ first and second consecutive pregnancies were included. Data on the mothers’ early-pregnancy weight, delivery weight, and the offspring's gestational age was extracted from the MBR, which covers more than 99% of all births in Sweden (Källén et al., Reference Källén, Källén and Otterblad Olausson2003). The twin mothers and their offsprings were identified in the MGR, while data on zygosity was collected from two surveys: SALT and STAGE, which are part of the STR (Furberg et al., Reference Furberg, Lichtenstein, Pedersen, Thornton, Bulik, Lerman and Sullivan2008; Lichtenstein et al., Reference Lichtenstein, Sullivan, Cnattingius, Gatz, Johansson, Carlstrom and Pedersen2006). Zygosity was assessed by classical and widely used questions on physical similarity that have shown high validity in previous studies using DNA (Lichtenstein et al., Reference Lichtenstein, Sullivan, Cnattingius, Gatz, Johansson, Carlstrom and Pedersen2006; Silventoinen et al., Reference Silventoinen, Magnusson, Tynelius, Kaprio and Rasmussen2008). Ethical approval for the study was granted by the Regional Ethics Committee, Stockholm, Sweden, and the participants in the cohorts included in the STR have given informed consent.
From the target population of 5,453 observations (pregnancies), the following observations were excluded (N = number of pregnancies): unknown zygosity (N = 803), implausible values on early-pregnancy weight (<35 kg), GWG (≤-21 kg or ≥36 kg), height (<130 cm), and offspring's gestational age (<week 30 or >week 44), and birth weight (<700 g; N = 16). Also, we excluded mothers who gave birth between 1982 and 1989 who had an early-pregnancy weight or delivery weight above 98 kg (during this period the MBR coded all values above 98 kg as 99 kg; N = 41). We dropped the twin mothers whose co-twin had not given birth to at least one child during the study period. Of the remaining 2,318 female twins, 1,388 individuals (380 MZ and 314 DZ complete twin pairs) had complete data on the first pregnancy, and 930 twin individuals (242 MZ and 223 DZ complete twin pairs) had complete data on the second pregnancy. Finally, 143 complete twin pairs (81 MZ and 62 DZ) had complete GWG data on both the first and the second pregnancies (subanalysis). When comparing the mean GWG of the first pregnancy for the excluded observations with unknown zygosity (N = 393) to the mean GWG of the study cohort, the results were fairly similar (13.8 kg and 14.0 kg respectively).
Measurements of Gestational Weight Gain and Covariates
The mothers’ GWG was defined by subtracting the weight at delivery (measured before and in the same gestational week as delivery) by early-pregnancy weight (measured at the first antenatal visit, occurring within the first 12 weeks of pregnancy for 90% of the women in the MBR; Källén et al., Reference Källén, Källén and Otterblad Olausson2003). Maternal height was based on self-reported data (recorded by the midwife at the first antenatal visit) and this variable was used together with early-pregnancy weight to calculate early-pregnancy body mass index (BMI, kg/m2). Parity, gestational age, and diseases during pregnancy (pre-eclampsia and gestational diabetes) were all measured or diagnosed by midwives, obstetricians, or other medical doctors as part of normal clinical practice. These data from the MBR have previously been evaluated as ‘acceptable’ to ‘good’ in terms of its accuracy and completeness (Cnattingius et al., Reference Cnattingius, Ericson, Gunnarskog and Källén1990). We also collected data from the MBR on maternal age at birth and smoking in early pregnancy (recorded at the first antenatal clinical assessment). For the majority of births since the 1980s, gestational age has been assessed by ultrasound scans (around gestational weeks 16–20 with an accuracy of ±7 days). If it could not be assessed by ultrasound scans, gestational age was calculated from the first day of the last menstrual period (LMP) (National Board of Health and Welfare, 2014).
The variables gestational age, maternal age at birth, and early-pregnancy BMI were included as covariates in both the uni- and bivariate models, based on previous research that has linked these variables to GWG (Prysak et al., Reference Prysak, Lorenz and Kisly1995; Rasmussen & Yaktine, Reference Rasmussen and Yaktine2009). Parity was used to define the mothers’ first and second pregnancies. The variables on diseases during pregnancy (pre-eclampsia and gestational diabetes) and smoking during pregnancy have also been associated with GWG (Haugen et al., Reference Haugen, Brantsaeter, Winkvist, Lissner, Alexander, Oftedal and Meltzer2014; Hedderson et al., Reference Hedderson, Gunderson and Ferrara2010; Nohr et al., Reference Nohr, Vaeth, Baker, Sorensen, Olsen and Rasmussen2009) and were used for the sensitivity analyses described below.
First, we assessed the nature of the genetic and environmental contributions to the variance in GWG by comparing intra-class correlations (ICCs) within MZ and DZ twin pairs. The ICCs were also adjusted for potential confounding as described below.
Based on the genetic resemblance and the equal environment assumption of twins, four sources of variation can be described using SEM: additive genetic (A), genetic dominance (D), common environment (C), and unique environment (E). To address potential confounding, the structural equation models were adjusted for gestational age, maternal age at birth, and early-pregnancy BMI by computing regression residuals in Stata 12.1 (Stata Corp, College Station, Texas, USA). All three covariates were statistically significant (p < .05). According to the general rule that if the ICCs are not more than twofold higher in MZ than DZ twins, dominance genetic effects do not play a role in the model. Although this rule suggests no dominance genetic effects according to the results of our ICCs, for completeness we fitted both ADE and ACE univariate models to our data for the two traits, and compared these models in terms of model fit. The genetic modeling was carried out using the Mplus statistical package (Muthén & Muthén, Reference Muthén and Muthén2010).
We also tested the technical assumptions of twin modeling (same means and variances in MZ and DZ twins, as well as in first and second twin within the pair), by carrying out χ2 tests. A non-significant p-value indicates that these assumptions are not violated. The fit of the models was evaluated using four different likelihood-based measures: (1) likelihood ratio test, (2) Akaike's information criterion (AIC), (3) Bayesian Information Criteria (BIC), and (4) root mean square error of approximation (RMSEA) (Nevitt & Hancock, Reference Nevitt and Hancock2000; Posada & Buckley, Reference Posada and Buckley2004). Typically, the models having the lowest AIC and/or BIC and/or RMSEA are considered to be the most parsimonious. We also carried out χ2 tests to examine the difference in model fit when comparing more parsimonious models to less parsimonious ones.
In order to assess covariance of additive genetic and/or unique environmental factors between GWG during the first and the second pregnancy, we also carried out a bivariate Cholesky decomposition analysis. This model decomposes the variance and the covariance into the latent factors A, C, and E to identify potential overlapping or distinct effects. To estimate the magnitude of the overlap between genes and environment that influence the two traits, we calculated genetic and environmental correlations (r A and r E) between the two pregnancies. The proportions of these additive genetic and unique environmental factors, which are shared between the two pregnancies, were calculated by squaring these correlations. For model fit and adjustments for covariates, we applied the same methods as described in the univariate analyses above.
Lastly, we planned to perform sensitivity analyses to examine if the inclusion of twin pairs with diseases or lifestyle patterns during pregnancy known to affect GWG (pre-eclampsia, gestational diabetes, and smoking during early pregnancy) affected the results, as well as the inclusion of mothers in the dataset with early-pregnancy or delivery weight >98 kg between the years 1982 and 1989. However, as no mothers were diagnosed with pre-eclampsia in our cohort, and only six individuals had gestational diabetes (adding both the first and the second pregnancies together), we were not able to carry out sensitivity analyses that included these diseases. The way in which we defined GWG was also assessed by using a different measure of GWG: relative GWG (GWG/gestational week). These results were then compared to the original results when using GWG per se, that is, delivery weight subtracted by early-pregnancy weight.
Descriptive statistics in terms of mean values of the participating twin mothers’ pregnancy and birth-related characteristics are shown in Table 1. On average, GWG was higher in the first pregnancy compared to the second, whereas early-pregnancy BMI was slightly higher in the second pregnancy compared to the first. Pearson correlations of GWG and each of the covariates are also displayed in Table 1, and the correlations were overall weak. ICCs were higher in MZ than DZ twin pairs for both pregnancies, although lower among MZ twins in the second pregnancy (Table 2). The adjusted models differed only marginally from the unadjusted ICC estimates.
N = number of twin individuals; MZ = monozygotic; DZ = dizygotic; BMI = body mass index.
aICCs were calculated using residuals from the regression models which were, in the adjusted model, adjusted for early-pregnancy BMI, gestational age, and maternal age. bNumber of twin pairs included in 1st pregnancy: MZ = 380, DZ = 314. cNumber of twin pairs included in 2nd pregnancy: MZ = 242, DZ = 223.
The overall univariate ACE model for the first pregnancy showed good fit to the data (χ2 test, p = .69, χ2 value = 3.9), that is, suggesting same means and variances in MZ and DZ twins according to the general assumptions of twin modeling. The χ2 test, which was used to check the overall ACE model fit of the second pregnancy, was significant (p = .01, χ2 value = 16.4), implying that the means of GWG in MZ and DZ twins differed to a larger extent than in the first pregnancy. As seen in Table 1, the mean GWG of the second pregnancy was somewhat lower in DZ twins compared to MZ twins (12.6 kg and 13.3 kg respectively); however, the difference was still modest.
As seen in Table 3, the univariate ADE and ACE models showed very similar results with regards to model fit in both pregnancies. In terms of the ACE model, SEM showed that the contribution of the shared environmental components (C) was small and non-significant in the first pregnancy (C = 0.03, 95% CI: 0.00, 0.26). The dominance genetic component (D) of the ADE models in both the first and the second pregnancy was zero, and this was therefore discarded. As a next step, we tested the model fit of both AE and CE models by constraining the shared environmental or additive genetic variance components to zero. These models were then compared to the ACE models by using the four likelihood measures explained above. In both the first and the second pregnancy, the AE models had a better fit compared to the ACE models (χ2 p value = .80 and .31 respectively, adjusted models). The CE model had worse fit than the AE model in the first pregnancy, whereas in the second pregnancy the results for the model fit for both AE and CE models were very similar. However, due to the larger sample size in the first pregnancy presumably providing more accurate results, we believe that additive genetic factors also play a role in the second pregnancy. The AE models were therefore chosen as the preferred models in both pregnancies.
P1 = first pregnancy; P2 = second pregnancy; A = additive genetic component; C = common environment component; E = unique environmental component; D = dominance genetic component; RMSEA = root means square error of approximation; BIC = Bayesian information criterion; AIC = Akaike information criterion. LL = log likelihood; χ2 = chi-square test p value. *best-fitting model. 1Number of twin pairs included in 1st pregnancy: MZ = 380 DZ = 314. 2Number of twin pairs included in 2nd pregnancy: MZ = 242, DZ = 223. 4The model was adjusted for early-pregnancy BMI, gestational age, and maternal age.
As seen in Table 3, under the best-fitting adjusted AE model, additive genetic factors (A) explained 43% of the variance in GWG of the first pregnancy (95% CI: 36–51%). The heritability estimate was lower in the mothers’ second pregnancy, where the additive genetic component explained approximately one-third of the variance in GWG (26%, 95% CI: 16–36%), and consequently the variance that remained was explained by unique environmental factors, including random measurement error (72%, 95% CI: 62–82%). Due to rather large uncertainty in the two heritability estimates, as shown by the confidence intervals, there was no evident difference in the genetic architecture of the two pregnancies in terms of additive genetic factors.
Cholesky Decomposition Analysis
Next, as a subanalysis, we analyzed the covariance between the twin mothers’ GWG in the first and second pregnancy using a bivariate Cholesky decomposition model. The overall phenotypic correlation between GWG in the first and second pregnancy was 0.46 (95% CI: 0.36, 0.54).
In terms of model fit, we started off comparing the AE model to the ACE model, as the ADE and CE models showed worse fit in the univariate models. When comparing the AE model to the ACE model, the ACE model was not found to be statistically significantly better than the AE model (χ2 test, –2 LL = 0.63, degrees of freedom [df] = 3, p = .89). This conclusion was also supported by the four different likelihood measures used (as described in the methods). When comparing a more parsimonious AE model by constraining A 2 to zero, that is, additive genetic factors specific to the second pregnancy only, with the full AE model, the change in the model fit was not statistically significant (χ2 test, –2 LL = 1.7, df = 1, p = .19). However, when fitting the AE model without constraining the A 2 component to zero, the coefficient estimate was 1.63 (95% CI: 0.44, 2.83), thus statistically significantly larger than zero. In light of this, we chose to use the AE model with an A 2 component.
As shown in Figure 1, the results from the best-fitting AE bivariate model suggest that there is an overlap (covariance) in both the genetic (A 1) and unique environmental (E 1) components, although specific unique environmental (E 2) and genetic factors (A 2) also seem to influence the variation in GWG in the second pregnancy. The variance explained by additive genetic factors in the bivariate model (see Table 4) seemed to be similar to the heritability estimates obtained from the univariate results, where the estimates were larger in the first pregnancy compared to the second (0.40 and 0.34 respectively). The covariance between GWG of the two pregnancies was to a larger extent explained by additive genetic factors (67%, 95% CI: 41%, 92%) compared to unique environmental factors (33%, 95% CI: 8%, 59%). The additive genetic correlation (r A) was 0.81 (95% CI: 0.55, 1.08), and the unique environmental correlation (r E) was 0.24 (95% CI: 0.06, 0.42). The squared correlations (r A 2 and r E 2) indicated that 66% of the genes and 6% of the unique environmental factors were shared between the two pregnancies.
GWG = gestational weight gain; V(GWG) = variance in gestational weight gain; A 1 = relative variance component explaining the additive genetic variance in GWG in the first pregnancy; A 2 = relative variance component explaining the additive genetic variance in GWG in the second pregnancy; E 1 = relative variance component explaining the unique environmental variance in GWG in the first pregnancy; E 2 = relative variance component explaining the unique environmental variance in GWG in the second pregnancy; Cov(GWG1, GWG2) = Covariance in gestational weight gain between the first and the second pregnancy.
As previously explained, we carried out two sensitivity analyses where we excluded mothers who at their first antenatal visit reported to be smokers (N = 386 in total, both first and second pregnancy) and mothers with an early-pregnancy or delivery weight of above 98 kg between the years 1982 and 1989 (N = 41 in total). None of the results differed from those presented earlier, neither in the univariate nor in the bivariate analyses. The observations from the first analysis where the mothers reported to be smokers were therefore kept in the analyses, and the latter 41 cases were excluded (as mentioned earlier, due to errors in the data). We also analyzed GWG as relative GWG (weight gain per gestational week); however, as there were very small changes in the results, GWG was kept as a continuous measure in kilograms.
In our large nation-wide register-based twin study, we showed that GWG was moderately heritable (ranging from approximately 30–40%), based on the best-fitting AE models of the twin mothers’ first and second pregnancies. A combination of environmental factors unique to each twin mother, and possibly also some random measurement error, explained the remaining part of the total variation in weight gain during pregnancy. As stated previously, due to the results from the ACE model (with a non-significant C component) in the first pregnancy, we cannot rule out that the AE models could also include a certain amount of shared environment. We also found that a larger part of the variation in GWG of the mothers’ second pregnancy was explained by unique environmental factors compared to the first pregnancy (72% and 57% respectively). It should also be mentioned that residual variation, such as potential measurement error in GWG (e.g., due to variability in measurement routines), is also a part of the unique environmental factors. Any potential measurement error should, however, be random within each twin pair. The findings from our bivariate model suggested that separate additive genetic factors influenced the mothers’ GWG in the second compared to the first pregnancy; however, it also suggested that there was a large overlap, where the same genetic factors influenced GWG in both the first and the second pregnancy. In fact, we found that 66% of the additive genetic factors were shared between the two pregnancies. A similar pattern was also found for the unique environmental variance, where unique environmental factors that influenced the GWG in the first pregnancy also influenced the GWG in the second pregnancy. However, the proportion to which these unique environmental factors were shared between the pregnancies was considerably smaller compared to the additive genetic factors (6%). In addition, there seemed to be non-shared environmental factors influencing the GWG only in the second pregnancy.
Earlier studies in the area of GWG have mainly focused on assessing the environmental determinants of GWG, hence very little is known about the genetic influences on GWG and its heritability (Dabelea et al., Reference Dabelea, Hanson, Lindsay, Pettitt, Imperatore, Gabir and Knowler2000; Ludwig & Currie, Reference Ludwig and Currie2010; Power & Jefferis, Reference Power and Jefferis2002). As previously mentioned, GWG is associated with offspring child and adult BMI (Cohen et al., Reference Cohen, Chaffee, Rehkopf, Coyle and Abrams2014; Reynolds et al., Reference Reynolds, Osmond, Phillips and Godfrey2010; Sridhar et al., Reference Sridhar, Darbinian, Ehrlich, Markman, Gunderson, Ferrara and Hedderson2014), and previous twin studies aiming to determine the heritability of growth-related traits have primarily studied the heritability of BMI. As shown in a recent systematic review and in large national twin studies, the heritability estimates of BMI are generally high (~50–80%) (Elks et al., Reference Elks, den Hoed, Zhao, Sharp, Wareham, Loos and Ong2012; Herskind et al., Reference Herskind, McGue, Sorensen and Harvald1996; Hjelmborg et al., Reference Hjelmborg, Fagnani, Silventoinen, McGue, Korkeila, Christensen and Kaprio2008; Maes et al., Reference Maes, Neale and Eaves1997; Schousboe et al., Reference Schousboe, Willemsen, Kyvik, Mortensen, Boomsma, Cornes and Harris2003) and fairly stable from early childhood into adulthood (Silventoinen et al., Reference Silventoinen, Pietilainen, Tynelius, Sorensen, Kaprio and Rasmussen2007). Despite a relatively high heritability, a recent international genome-wide association study (GWAS) by Locke et al. (Reference Locke, Kahali, Berndt, Justice, Pers, Day and Speliotes2015) found that the 97 loci that were associated with BMI explained less than 3% of the total variation in BMI. This indicates that even in the largest GWAS to date, only a small proportion of the genetic variation can be explained by specific genes. As our findings suggest that GWG has a moderate heritability, it is perhaps not surprising that many previous studies (although not genome-wide) have had difficulties in locating genes or genetic variants related to the trait (Dishy et al., Reference Dishy, Gupta, Landau, Xie, Kim, Smiley and Stein2003; Lawlor et al., Reference Lawlor, Fraser, Macdonald-Wallis, Nelson, Palmer, Davey Smith and Tilling2011; Stuebe et al., Reference Stuebe, Lyon, Herring, Ghosh, Wise, North and Siega-Riz2010). In addition, as GWG includes several other components — for example, the fetus, amniotic fluid, and placenta (Butte et al., Reference Butte, Ellis, Wong, Hopkinson and Smith2003) — than the maternal increase in adipose tissue, it is likely influenced by other genetic or biological pathways than the general ones related to adiposity.
The pregnancy period is often referred to as a ‘window of opportunity’ in terms of changing lifestyle factors influencing the long-term health of both the mother and child (Shapira, Reference Shapira2008). Our results support this idea by showing that environmental factors explain more than the majority of the variation of GWG. We also observed that the heritability estimate was lower in the mothers’ second pregnancy compared to the first. A possible biological mechanism behind this lower estimate could be that the physiological adaptions of the female body during pregnancy play a larger role during the first pregnancy compared to following pregnancies. However, as the estimates did not differ significantly from each other, this finding might also be incidental. Larger twin studies are needed to replicate our results as well as to assess the heritability of GWG in several consecutive pregnancies to see whether the magnitude of the environmental influence stays constant after the first pregnancy.
We observed that both overlapping and distinct genetic and unique environmental factors influenced the GWG in the first and the second pregnancy. In terms of the genetic effects, the findings could be explained by changes in the mothers’ gene expression; for example, due to epigenetic modifications from one pregnancy to the next. It is also possible that there are genes being switched on or off after the mother's first pregnancy, which could result in partially different genes playing a role in the GWG of the second pregnancy compared to the first. Regarding the non-shared environmental factors, it seems plausible that lifestyle factors that influence GWG during the first pregnancy (e.g., maternal dietary intake known to be associated with GWG; Rasmussen & Yaktine, Reference Rasmussen and Yaktine2009) can also influence GWG of the second pregnancy. On the same note, there seem to be unique environmental factors that influence the mothers’ GWG only during the second pregnancy, suggesting that different environmental factors can also influence the variation in GWG in the second compared to the first pregnancy. It is, however, important to mention that the sample size of the bivariate analysis was rather small. Therefore, the results should be interpreted with caution and need replication in future larger twin studies or similar family-based studies.
The main strength of our study lies in its population-based nature and its rather large sample (taking into account the fact that we have unique register-based data on GWG), which further offered the opportunity to estimate and compare the heritability of two consecutive pregnancies. As the study is register-based, we also had objectively measured data on GWG and on all covariates apart from maternal height (which was used to calculate early-pregnancy BMI). Although self-reported height is prone to overestimation, we find it unlikely that such errors would cause over- or underestimation of our adjusted estimates as the magnitude of the overestimation has been reported to be small and the validity high (Brunner Huber, Reference Brunner Huber2007). With regards to generalizing our results to singleton mothers, twins have been shown to be representative of the general population for most traits after early childhood (Kaprio & Silventoinen, Reference Kaprio and Silventoinen2011). Our study is also afflicted with some limitations. In terms of external validity, our findings may be limited to populations in similar developed countries and to women with early-pregnancy or delivery weight <99 kg. Even though our sample size is rather large, as mentioned in the results section, the C component of the adjusted ACE model was small and non-significant (0.03, 95% CI: 0.00, 0.26). This could indicate that we lack power to detect a possible role of shared environmental factors, as well as making it difficult to distinguish between the familial factors A and C. As previously stated, larger population-based twin studies are needed to replicate our findings.
In conclusion, our study suggests that GWG is moderately heritable. The largest part of the variation in the trait was explained by environmental factors unique to each twin. This is of importance for clinicians and public health authorities working to prevent excessive pregnancy weight gain, as it is currently only the environmental factors that can be influenced by, for example, public health initiatives and primary prevention.
Information on zygosity was kindly provided by the Swedish Twin Registry, which was supported by a grant from the Ministry for Higher Education. This study was supported by a grant to Finn Rasmussen from the Swedish Research Council for Health, Working Life and Welfare (FORTE) (Grant number: 2010-0671) and Karolinska Institutet Faculty Funds (KID; Grant number: 6549/2010-225). The funding organizations had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.