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Reduced embryo sensitivity to abscisic acid in a sprouting-susceptible sorghum (Sorghum bicolor) variety is associated with altered ABA signalling

Published online by Cambridge University Press:  01 June 2007

Nicolás Gualano ,
Fernando Carrari ,
María Verónica Rodríguez ,
Laura Pérez-Flores ,
Rodolfo Sánchez ,
Norberto Iusem  and
Roberto Benech-Arnold
Nicolás Gualano
Affiliation:
Cátedra de Cerealicultura-IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Av. San Martín 4453 (C1417DSE), Buenos, Aires, Argentina
Fernando Carrari
Affiliation:
INTA Castelar, CONICET, Argentina
María Verónica Rodríguez
Affiliation:
Cátedra de Cerealicultura-IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Av. San Martín 4453 (C1417DSE), Buenos, Aires, Argentina
Laura Pérez-Flores
Affiliation:
Departamento de Ciencias de la Salud, DCBS, Universidad Autónoma Metropolitana-Iztapalapa, D.F., México
Rodolfo Sánchez
Affiliation:
Cátedra de Fisiología Vegetal, IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, Argentina
Norberto Iusem
Affiliation:
Laboratorio de Fisiología y Biología Molecular, FCEyN, Universidad de Buenos Aires, Ciudad Universitaria, Argentina
Roberto Benech-Arnold*
Affiliation:
Cátedra de Cerealicultura-IFEVA, Facultad de Agronomía, Universidad de Buenos Aires, CONICET, Av. San Martín 4453 (C1417DSE), Buenos, Aires, Argentina
*
*Correspondence Fax: +54 11 4524 8039 (ext. 33) Email: benech@ifeva.edu.ar
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Abstract

In the work reported in this paper, we attempted to elucidate the nature of the different abscisic acid (ABA) sensitivities presented by developing embryos from sorghum [Sorghum bicolor (L.) Moench] lines with contrasting pre-harvest sprouting (PHS) behaviour (Redland B2, susceptible; IS 9530, resistant). We explored two different hypotheses for a possible mechanism: (1) a different functionality of the ABA signalling pathway, and (2) a different rate of ABA degradation/conjugation in the apoplast of embryos from these genotypes. To assess the first possibility, we used an ABA-responsive gene (Rab17) as a reporter of changes in endogenous ABA content, which were artificially induced in embryos from both genotypes by means of fluridone application immediately after anthesis, to reduce ABA content, and embryo incubation in the presence of ABA. A defect in ABA signalling should be seen as a level of Rab17 expression that is independent of endogenous ABA content. For testing the second possibility at two stages of development, embryos from both lines were isolated and incubated in water for different periods. ABA concentrations in embryos and the incubation media were quantified through radioimmunoassay. In contrast to our findings for the resistant IS 9530 line, Rab17 expression did not respond to changes in ABA levels in sensitive Redland B2 embryos. The ABA degradation/conjugation rates in embryos and incubation media did not show clear differences between sorghum lines for any of the developmental stages analysed. These results suggest that a disruption in the ABA signal transduction pathway in Redland B2 underlies the low ABA sensitivity shown by embryos from this line.

Keywords

abscisic acidabscisic acid sensitivityabscisic acid signallinggerminationpre-harvest sproutingseed dormancySorghum bicolor
Type
Research Article
Information
Seed Science Research , Volume 17 , Issue 2 , June 2007 , pp. 81 - 90
Copyright
Copyright © Cambridge University Press 2007

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