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Evidence of different compounds in smoke derived from legumes and grasses acting on seed germination and seedling emergence

Published online by Cambridge University Press:  19 April 2017

Lei Ren ,
Yuguang Bai  and
Martin Reaney
Lei Ren
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada
Yuguang Bai*
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada
Martin Reaney
Affiliation:
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, S7N 5A8, Canada
*
*Correspondence E-mail: yuguang.bai@usask.ca
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Abstract

Our previous study showed that smoke derived from alfalfa (Medicago sativa) caused different germination responses compared with that from prairie hay (Festuca hallii) and wheat straw (Triticum aestivum), but the mechanism remained unclear. In this study, we used Salad Bowl lettuce (Lactuca sativa) as a quick bioassay to trace the active compounds in each of these three smoke solutions. Column chromatography and high performance liquid chromatography (HPLC) were used to separate and identify active fractions. Seeds of four species from Fescue Prairie were primed for 24 h at room temperature in darkness using serial dilutions of separated active fractions, as well as karrikinolide (KAR1). After priming, seeds were dried at room temperature in darkness for 7 days and subsequently incubated at 10/0°C or 25/15°C in 12 h light–12 h dark or 24 h darkness for 49 days. KAR1 was in the smoke made from prairie hay, and wheat straw, but was absent in alfalfa smoke. Priming in KAR1 solutions increased germination of three native species. Priming in highly concentrated KAR1 reduced radicle length of Cirsium arvense, the only non-native species. Even though KAR1 has the potential to enhance regeneration of native species in the Fescue Prairie, KAR1 is not universally present in smoke derived from different plant materials. Unknown compound(s) in smoke derived from legumes remain to be identified.

Keywords

Festuca halliigerminationkarrikinolideMedicago sativaseedling growthTriticum aestivum
Type
Research Papers
Information
Seed Science Research , Volume 27 , Special Issue 2: Seed Ecology , June 2017 , pp. 154 - 164
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Copyright
Copyright © Cambridge University Press 2017 

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