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Endo-β-mannanase activity and seed germination of thermosensitive and thermotolerant lettuce genotypes in response to seed priming

Published online by Cambridge University Press:  22 February 2007

Warley M. Nascimento ,
Daniel J. Cantliffe  and
Donald J. Huber
Warley M. Nascimento
Affiliation:
University of Florida, IFAS, Horticultural Sciences Department, 1251 Fifield Hall, PO Box 110690, Gainesville, FL 32611-0690, USA
Daniel J. Cantliffe*
Affiliation:
University of Florida, IFAS, Horticultural Sciences Department, 1251 Fifield Hall, PO Box 110690, Gainesville, FL 32611-0690, USA
Donald J. Huber
Affiliation:
University of Florida, IFAS, Horticultural Sciences Department, 1251 Fifield Hall, PO Box 110690, Gainesville, FL 32611-0690, USA
*
*Correspondence Fax: 352-392-9905 Email: djc@mail.ifas.ufl.edu
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Abstract

The effects of seed priming on germination and endo-β-mannanase activity at inhibitory and non-inhibitory temperatures in thermosensitive ‘Dark Green Boston’ (DGB) and in thermotolerant ‘Everglades’ (EVE) lettuce were investigated. A single-seed endo-β-mannanase assay was used to follow the enzyme activity during priming. Seeds were primed at 15°C in aerated solutions of polyethylene glycol (PEG) with constant light, then redried. Primed and non-primed seeds germinated 100% at 20°C. At 35°C, non-primed and primed EVE seeds germinated 100%, whereas non-primed seeds of DGB germinated only 4%. During priming, endo-β-mannanase activity increased between 24 and 48 h in EVE and between 24 and 72 h in DGB after the beginning of osmotic imbibition. Endo-β-mannanase activity persisted in primed seeds following seed drying, was detected before radicle protrusion and was present in the micropylar region in front of the radicle tip. Higher enzyme activity was observed in primed seeds and EVE compared with non-primed and DGB seeds. The results suggest that priming may overcome the inhibitory effect of high temperature in thermosensitive lettuce seeds due to increased endo-β-mannanase activity, possibly leading to a weakening of endosperm, thus overcoming thermodormancy.

Keywords

Lactuca sativaendo-β-mannanaseendosperm weakeningosmotic conditioningseed primingthermodormancythermoinhibition
Type
Research Article
Information
Seed Science Research , Volume 11 , Issue 3 , September 2001 , pp. 255 - 264
Copyright
Copyright © Cambridge University Press 2001

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