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Effect of various sugars and polyols on the tolerance to desiccation and freezing of oil palm polyembryonic cultures

Published online by Cambridge University Press:  19 September 2008

D. Dumet ,
F. Engelmann ,
N. Chabrillange ,
S. Dussert  and
Y. Duval
D. Dumet
Affiliation:
ORSTOM, 911, av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
F. Engelmann*
Affiliation:
ORSTOM, 911, av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
N. Chabrillange
Affiliation:
ORSTOM, 911, av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
S. Dussert
Affiliation:
ORSTOM, 911, av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
Y. Duval
Affiliation:
ORSTOM, 911, av. Agropolis, BP 5045, 34032 Montpellier Cédex 01, France
*
* Correspondence
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Abstract

During a 7-day conditioning treatment of clumps of oil palm (Elaeis guineensis Jacq.) somatic embryos on a medium containing 0.75 M sucrose, the fructose and glucose concentrations remained constant, whereas a ten-fold and twenty-fold increase were noted for sucrose and starch concentrations, respectively. The only new sugar detected was arabinose which remained at a low concentration. After conditioning on media supplemented with various sugars and polyols at similar osmolarities, recovery of clumps of embryo growth was satisfactory except with ribose. After an additional desiccation period, survival was optimal with fructose, galactose, sucrose and glucose, intermediate with maltose and lower with other compounds. When embryos were cryopreserved without previous desiccation, survival was noted after conditioning treatment with sucrose only. In contrast, when freezing was performed after dehydration, survival could be obtained with several substances. It was optimal with sucrose, fructose, galactose and raffinose but was possible also with sorbitol and glucose. Intensity of recovery of proliferation was highest with embryos conditioned with sucrose.

Keywords

oil palmclumps of somatic embryossugarspolyolsdesiccationcryopreservation
Type
Research Papers
Information
Seed Science Research , Volume 4 , Issue 3 , September 1994 , pp. 307 - 313
Copyright
Copyright © Cambridge University Press 1994

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References

Amuti, K.S. and Pollard, M. (1977) Soluble carbohydrates of dry and developing seeds. Phytochemistry,1, 16–529.Google Scholar
Anandarajah, K. and McKersie, B.D. (1992) Influence of plating density, sucrose and light during development on the germination and vigour of Medicago sativa L. somatic embryos after desiccation. Seed Science Research 1, 2–133.Google Scholar
Blackman, S.A., Obendorf, R.L. and Leopold, A.C. (1992) Maturation proteins and sugars in desiccation tolerance of developing soybean seeds. Plant Physiology 1, 100–225.Google Scholar
Cram, W.J. (1984) Mannitol transport and suitability as an osmoticum in root cells. Physiologia Plantarum 1, 61–396.Google Scholar
Crowe, J.H. and Crowe, L.M. (1986) Stabilisation of membranes in anhydrobiotic organisms. pp 188–209in Leopold, A.E. (Ed.) Membranes, metabolism and dry organisms. Ithaca, New York, Comstock Publishing Associates.Google Scholar
Dumet, D., Engelmann, F., Chabrillange, N. and Duval, Y. (1993a) Cryopreservation of standard oil palm (Elaeis guineensis Jacq.) somatic embryos involving a desiccation step. Plant Cell Reports 1, 12–352.Google Scholar
Dumet, D., Engelmann, F., Chabrillange, N. and Duval, Y. (1993b) Development of cryopreservation for oil palm somatic embryos using an improved process. Oléagineux 1, 48–273.Google Scholar
Dumet, D., Engelmann, F., Chabrillange, N. and Duval, Y. (1993c) Importance of sucrose for the acquisition of tolerance to desiccation and cryopreservation of oil palm somatic embryos. Cryo-Letters 1, 14–243.Google Scholar
Engelmann, F., Duval, Y. and Dereuddre, J. (1985) Survie et prolifération d'embryons somatiques de palmier à huile (Elaeis guineensis Jacq.) après congélation dans l'azote liquide. Comptes Rendus de l'Académie des Sciences Paris, Série III 301, 111–116.Google Scholar
Farrant, J.M., Pammenter, N.W. and Berjak, P. (1992) A contribution to an understanding of desiccation tolerance from a study of a desiccation sensitive seed species. pp 715–722in Côme, D. and Corbineau, F. (Eds) Fourth international workshop on seeds, basic and applied aspects of seed biology, Vol. 3, ASFIS Paris.Google Scholar
Finkle, B.J., Zavala, M.E. and Ulrich, J.M. (1985) Cryoprotective compounds in the viable freezing of plant tissues. pp 75–114in Kartha, K.K. (Ed.) Cryopreservation of plant cells and organs, CRC Press, Boca Raton Florida.Google Scholar
Keuls, M. (1952) The use of studentised range in connexion with analysis of variance. Euphytica 1, 1–112.CrossRefGoogle Scholar
Koster, K. and Leopold, A.C. (1988) Sugars and desiccation tolerance in seeds. Plant Physiology 1, 88–829.Google Scholar
Murashige, T. and Skoog, F. (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 1, 15–473.Google Scholar
Newman, D. (1939) The distribution of ranges in samples from a normal distribution expressed in terms of an independent estimate of standard deviation. Biometrika 31, 20–30.CrossRefGoogle Scholar
Pannetier, C., Arthuis, P. and Liévoux, D. (1981) Néoformation de jeunes plantes d'Elaeis guineensis à partir de cals primaires obtenus sur fragments foliaires cultivés in vitro. Oléagineux 1, 36–119.Google Scholar
Ryan, T.A. (1960) Significance tests for multiple comparison of proportions, variances and other statistics. Psychological Bulletin, 1, 57–318.Google Scholar
Sagishima, K., Kubota, K. and Ashihara, H. (1989) Uptake and metabolism of sugars by suspension-cultured Catharanthus roseus cells. Annals of Botany 1, 64–185.Google Scholar
Slawinska, J. and Obendorf, R.L. (1991) Soybean somatic embryo maturation: composition, respiration and water relations. Seed Science Research 1, 1–251.CrossRefGoogle Scholar
Stranzel, M., Sjolund, R.D. and Komor, E. (1988a) Transport of glucose, fructose and sucrose by Streptanthus tortuosus suspension cells. I. Uptake at low sugar concentrations. Planta 1, 174–201.Google Scholar
Stanzel, M., Sjolund, R.D. and Komor, E. (1988b) Transport of glucose, fructose and sucrose by Streptanthus tortuosus suspension cells. I.I Uptake at high sugar concentrations. Planta 1, 174–210.Google Scholar
Strauss, G. and Hauser, H. (1986) Stabilization of lipid bilayer vesicles by sucrose during freezing. Proceedings of the National Academy of Sciences, USA 1, 83–2422.Google Scholar
Tessereau, H. (1993) Développement d'une méthode simplifiée de cryoconservation de tissus et d'embryons somatiques végétaux et étude de l'acquisition de la tolérance à la congélation. Thèse de Doctorat d'Université, Paris 6, France.Google Scholar
Williams, R.J. and Leopold, A.C. (1989) The glassy state in corn embryos. Plant Physiology 1, 89–977.Google Scholar
Withers, L.A. (1992) In vitro conservation. pp 169–200in Hammerschlag, F.A. and Litz, R.E. (Eds) Biotechnology in agriculture No. 8. Wallingford, CAB International.Google Scholar
Xu, N., Coulter, K.M. and Bewley, J.D. (1990) Absisic acid and osmoticum prevent germination of developing alfalfa embryos, but only osmoticum maintains the synthesis of developmental proteins. Planta 1, 182–382.Google Scholar