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Desiccation-induced dormancy in papaya (Carica papaya L.) seeds is alleviated by heat shock

Published online by Cambridge University Press:  22 February 2007

Christopher B. Wood ,
Hugh W. Pritchard  and
Dilip Amritphale
Christopher B. Wood
Affiliation:
Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex RH17 6TN, UK
Hugh W. Pritchard*
Affiliation:
Seed Conservation Department, Royal Botanic Gardens, Kew, Wakehurst Place, Ardingly, West Sussex RH17 6TN, UK
Dilip Amritphale
Affiliation:
Institute of Environmental Management and Plant Sciences, Vikram University, Ujjain, 456010 Madhya Pradesh, India
*
*Correspondence Fax: +44 (0)1444 894110 Email: h.pritchard@rbgkew.org.uk
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Abstract

The effects of desiccation and temperature on the germination capability of Carica papaya L. were investigated for seeds extracted from three commercial fruit batches. More than 50% of freshly isolated, cleaned (sarcotesta removed) but undried seeds germinated at 26°C. However, desiccation to approx. 20%seed RH reduced germination at this temperature to < 10%. A substantial increase in germination at alternating temperatures (33/19°C) indicated that desiccation induced seed dormancy rather than viability loss. Dormancy could be removed in a large proportion of the population by the application of a single heat shock to rehydrated seeds for 4 h at 36°C, with subsequent return to 26°C for germination. Longer (days) and shorter (minutes) periods of heat shock were less effective for releasing dormancy. Heat shock was generally applied 5 or 14 d after rehydration had started, but the treatment was equally effective after imbibition for only 1 d. Light was always applied during both imbibition and the post-heat shock treatment, but was not essential during the actual heat shock treatment. Rehydration and post-heat shock temperature treatments in the range of 16°C to 36°C revealed the same optima of 26°C. Dormancy was re-imposed in heat-shocked seeds when they were subsequently dried to seed relative humidities of 25 to 75%(5 to 11% moisture content [fresh weight basis]), but this state could be removed by a further heat shock. The longer heat-shocked seeds were held on agar-water at 26°C prior to re-drying, the greater the level of desiccation intolerance.

Keywords

Carica papaya L.seed dormancydesiccationheat shockgermination
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 2 , June 2000 , pp. 135 - 145
Copyright
Copyright © Cambridge University Press 2000

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References

Allen, G.S. (1962) Factors affecting the viability and germination behaviour of coniferous seed. VI. Stratification and subsequent treatment, Pseudotsuga menziesii (Mirb.) Franco. Forestry Chronicle 38, 485–496.Google Scholar
Baskin, C.C. and Baskin, J.M. (1998) Seeds. Ecology, biogeography, and evolution of dormancy and germination. San Diego, Academic Press.Google Scholar
Beardsell, D. and Mullett, J. (1984) Seed germination of Eucalyptus pauciflora Sieb. ex Spreng. from low and high altitude populations in Victoria. Australian Journal of Botany 32, 475–480.CrossRefGoogle Scholar
Chacko, E.K. and Singh, R.N. (1966) The effect of gibberellic acid on the germination of papaya seeds and subsequent seedling growth. Tropical Agriculture (Trinidad) 43, 341–346.Google Scholar
Conner, A.J. and Conner, L.N. (1988) Germination and dormancy of Arthropodium cirratum seeds. New Zealand Natural Sciences 15, 3–10.Google Scholar
Crawley, M.J. (1993) GLIM for ecologists. Oxford, Blackwell Scientific Publications.Google Scholar
Ellis, R.H., Hong, T.D. and Roberts, E.H. (1991) Effect of storage temperature and moisture on the germination of papaya seeds. Seed Science Research 1, 69–72.CrossRefGoogle Scholar
Felippe, G.M., Gherardi, E., Penteado, L.B.K., Annes, V.C.S. and Sene, C.M. (1970) Detecção de giberelinas durante a germinação de Rumex obtusifolius L. Arquivos do Instituto Biologico Sao Paulo 37, 177–187.Google Scholar
Helm, K.W. and Abernathy, R.H. (1990) Heat shock proteins and their mRNAs in dry and early imbibing embryos of wheat. Plant Physiology 93, 1626–1633.CrossRefGoogle ScholarPubMed
Hu, Y.M., Butcher, P.D., Sole, K., Mitchison, D.A. and Coates, A.R.M. (1998) Protein synthesis is shutdown in dormant Mycobacterium tuberculosis and is reversed by oxygen or heat shock. FEMS Microbiology Letters 158, 139–145.CrossRefGoogle ScholarPubMed
International Seed Testing Association (1999) International rules of seed testing, 1999. Seed Science and Technology 27, Supplement, Rules, 1999.Google Scholar
Jones, S.K., Gosling, P.G. and Ellis, R.H. (1998) Reimposition of conditional dormancy during air-dry storage of pre-chilled Sitka spruce seeds. Seed Science Research 8, 113–122.Google Scholar
Kimpel, J.A., Nagao, R.T., Goekjian, V. and Key, J.L. (1990) Regulation of the heat shock response in soybean seedlings. Plant Physiology 94, 988–995.CrossRefGoogle ScholarPubMed
Kovach, D.A. and Bradford, K.J. (1992) Imbibitional damage and desiccation tolerance of wild rice (Zizania palustris) seeds. Journal of Experimental Botany 43, 747–757.Google Scholar
Leopold, A.C., Glenister, R. and Cohn, M.A. (1988) Relationship between water content and afterripening in red rice. Physiologia Plantarum 74, 659–662.CrossRefGoogle Scholar
Leopold, A.C. and Vertucci, C.W. (1989) Moisture as a regulator of physiological reaction in seeds. pp. 51–67in Stanwood, P.C.; McDonald, M.B. (Eds) Seed moisture. Madison, Crop Science Society of America, Inc.Google Scholar
Lu, Y-T., Dharmasiri, M.A.N. and Harrington, H.M. (1995) Characterization of a cDNA encoding a novel heat-shock protein that binds to calmodulin. Plant Physiology 108, 1197–1202.Google Scholar
Magill, W., Deighton, N., Pritchard, H.W., Benson, E.E. and Goodman, B.A. (1994) Physiological and biochemical studies of seed storage parameters in Carica papaya. Proceedings of the Royal Society of Edinburgh 102B, 439–442.Google Scholar
Murdoch, A.J., Roberts, E.H. and Goedert, C.O. (1989) A model for germination responses to alternating temperatures. Annals of Botany 63, 97–111.CrossRefGoogle Scholar
Neumann, K., Dröge-Laser, W., Köhne, S. and Broer, I. (1997) Heat treatment results in loss of transgene-encoded activities in several tobacco lines. Plant Physiology 115, 939–947.CrossRefGoogle ScholarPubMed
Paz, L. and Vásquez-Yanes, C. (1998) Comparative seed ecophysiology of wild and cultivated Carica papaya trees from a tropical rain forest region in Mexico. Tree Physiology 18, 277–280.CrossRefGoogle ScholarPubMed
Pérez, A., Reyes, M.N. and Cuevas, J. (1980) Germination of two papaya varieties: effect of seed aeration, K-treatment, removing the sarcotesta, high temperature, soaking in distilled water, and age of seeds. Journal of Agriculture of the University of Puerto Rico 64, 173–180.Google Scholar
Pritchard, H.W. (1991) Water potential and embryonic axis viability in recalcitrant seeds of Quercus rubra. Annals of Botany 67, 43–49.Google Scholar
Reddy, R.K., Chaudhary, S., Patil, P. and Krishna, P. (1998) The 90 kDa heat shock protein (hsp90) is expressed throughout Brassica napus seed development and germination. Plant Science 131, 131–137.CrossRefGoogle Scholar
Rivero, F. and Cerdá-Olmedo, E. (1987) Spore activation by acetate, propionate and heat in Phycomyces mutants. Molecular and General Genetics 209, 149–153.CrossRefGoogle ScholarPubMed
Roberts, E.H. and Ellis, R.H. (1989) Water and seed survival. Annals of Botany 63, 39–52.CrossRefGoogle Scholar
Roberts, E.H., King, M.W. and Ellis, R.H. (1984) Recalcitrant seeds: their recognition and storage. pp. 38–52in Holden, J.H.W.; Williams, J.T. (Eds) Crop genetic resources: conservation and evaluation. London, George Allen and Unwin.Google Scholar
Ross, J.D. (1996) Dormancy breakage by chilling: phytochrome, calcium and calmodulin. pp. 157–169in Lang, G.A. (Ed) Plant dormancy. Physiology, biochemistry and molecular biology. Wallingford, UK, CAB International.Google Scholar
Roy, S. and Raghavan, V. (1994) Control of heat-shock response of young gametophytes of the sensitive fern is at the translational level. Journal of Experimental Botany 45, 477–483.CrossRefGoogle Scholar
Steadman, K.J., Pritchard, H.W. and Dey, P.M. (1996) Tissue-specific soluble sugars in seeds as indicators of storage category. Annals of Botany 77, 667–674.Google Scholar
Taylorson, R.B. and DiNola, L. (1989) Increased phytochrome responsiveness and a high-temperature transition in barnyardgrass (Echinochloa crus-galli) seed dormancy. Weed Science 37, 335–338.CrossRefGoogle Scholar
Trofimova, M.S., Andreev, I.R. and Kuznetsov, V.V. (1999) Calcium is involved in regulation of the synthesis of HSPs in suspension-cultured sugar beet cells under hyperthermia. Physiologia Plantarum 105, 67–73.Google Scholar
van Breusegem, F., Dekeyser, R., Garcia, A.B., Claes, B., Gielen, J., van Montagu, M. and Caplan, A.B. (1994) Heat-inducible rice hsp82 and hsp70 are not always coregulated. Planta 193, 57–66.Google Scholar
Vásquez-Yanes, C. and Orozco-Segovia, A. (1996) Comparative longevity of seeds of five tropical rain forest woody species stored under different moisture conditions. Canadian Journal of Botany 74, 1635–1639.CrossRefGoogle Scholar
Vierling, E. (1991) The roles of heat shock proteins in plants. Annual Review of Plant Physiology and Plant Molecular Biology 42, 579–620.CrossRefGoogle Scholar
Washitani, I. and Takenaka, A. (1987) Gap-detecting mechanism in the seed germination of Mallotus japonicus (Thunb.) Muell. Arg., a common pioneer tree of secondary succession in temperate Japan. Ecological Research 2, 191–201.Google Scholar
Westra, R.N. and Loomis, W.E. (1966) Seed dormancy in Uniola paniculata. American Journal of Botany 53, 407–411.Google Scholar