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ACC-N-Malonyltransferase activity during zygotic embryogenesis and germination of chick-pea seeds

Published online by Cambridge University Press:  22 February 2007

Clemente Martin-Remesal ,
Maria del Carmen Gomez-Jimenez  and
Angel J. Matilla
Clemente Martin-Remesal
Affiliation:
Departament de Biologia Vegetal, Laboratorio de Fisiologia Vegetal, Facultad de Ciencias, Universidad de Granda, 18001-Granada, Spain
Maria del Carmen Gomez-Jimenez
Affiliation:
Departamenta de Biologia Vegetal, Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706-Santiago de Compostela, La Coruna, Spain
Angel J. Matilla*
Affiliation:
Departamenta de Biologia Vegetal, Laboratorio de Fisiologia Vegetal, Facultad de Farmacia, Universidad de Santiago de Compostela, 15706-Santiago de Compostela, La Coruna, Spain
*
*Fax: +34-981-593-054 Email: bvmatilla@usc.es
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Abstract

Some physiological characteristics of ACC-Nmalonyltransferase (ACC-N-MTase) have been studied in the seeds of chick-pea (Cicer arietinum L.). This enzymatic activity was detectable during all periods of zygotic embryogenesis; however, the highest values were found in the dry seed. In dry seeds, the enzymatic activity was greater in the embryonic axis than in the cotyledons. During the onset of germination, activity increased more strongly in the cotyledons than in the embryonic axis, reaching its highest values in the storage organs coinciding with radicle emergence (18 to 24 h). Removal of the cotyledons strongly diminished enzymatic activity in the embryonic axis. Low concentrations of ethrel (50 µM) stimulated the axis ACC-N-MTase. The highest specific activity was found in the apical meristem of the embryonic axis, declining over the length of the organ. The role of ACC-N-MTase activity in the germinative process is discussed, together with the regulatory effect of ethylene on this ACCconjugating enzyme.

Keywords

ACC-N-malonyltransferaseCicer arietinumchick-peaembryogenesisethylenegerminationLeguminosae
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 1 , March 2000 , pp. 71 - 76
Copyright
Copyright © Cambridge University Press 2000

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