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The regulation of post-germinative transition from the cotyledon- to vegetative-leaf stages by microRNA-targeted SQUAMOSA PROMOTER-BINDING PROTEIN LIKE13 in Arabidopsis

Published online by Cambridge University Press:  18 March 2010

Ruth C. Martin
Affiliation:
USDA-ARS, National Forage Seed Production Research Center, Corvallis, Oregon97331, USA
Masashi Asahina
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Po-Pu Liu
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Jessica R. Kristof
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Jennifer L. Coppersmith
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Wioletta E. Pluskota
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
George W. Bassel
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Natalya A. Goloviznina
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Theresa T. Nguyen
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Cristina Martínez-Andújar
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
M.B. Arun Kumar
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Piotr Pupel
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
Hiroyuki Nonogaki*
Affiliation:
Department of Horticulture, Oregon State University, Corvallis, OR97331, USA
*
*Correspondence Fax: +1 (541) 737-3479 Email: hiro.nonogaki@oregonstate.edu

Abstract

Germination and early seedling development are critical for successful stand establishment of plants. Following germination, the cotyledons, which are derived from embryonic tissue, emerge from the seed. Arabidopsis seedlings at post-germinative stages are supported mainly by the supply of nutrition from the cotyledons until vegetative leaves emerge and initiate photosynthesis. The switch to autotrophic growth is a significant transition at the post-germinative stage. Here, we provide evidence that down-regulation of SQUAMOSA PROMOTER-BINDING PROTEIN LIKE13 (SPL13) by microRNA156 (miR156) plays an important role in the regulation of the post-germinative switch from the cotyledon stage to the vegetative-leaf stage. Silent mutations created in the SPL13 sequence in the region that is complementary to the miR156 sequence caused the deregulation of the mutant form of SPL13 (mSPL13) mRNA from miR156. Mutant seedlings over-accumulated miRNA-resistant messages and exhibited a delay in the emergence of vegetative leaves compared to wild-type seedlings. The delay was not observed in control transgenic plants expressing non-mutated SPL13, indicating that the phenotype was caused specifically by the silent mutations and deregulation of SPL13 from miR156. Characterization of the SPL13 promoter indicated that this gene is expressed mainly in the hypocotyl and affects leaf primordium development. These results suggest that the repression of SPL13 by miR156 is essential for normal post-germinative growth in Arabidopsis.

Type
Research Article
Copyright
Copyright © Cambridge University Press 2010

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The regulation of post-germinative transition from the cotyledon- to vegetative-leaf stages by microRNA-targeted SQUAMOSA PROMOTER-BINDING PROTEIN LIKE13 in Arabidopsis
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