Hostname: page-component-788cddb947-wgjn4 Total loading time: 0 Render date: 2024-10-07T23:55:05.273Z Has data issue: false hasContentIssue false

Proteomic analysis of the enhancement of seed vigour in osmoprimed alfalfa seeds germinated under salinity stress

Published online by Cambridge University Press:  16 April 2013

Rafika Yacoubi*
Affiliation:
Laboratoire de Biologie et Physiologie Cellulaires Végétales, Département de Biologie, Université de Tunis, Tunisia
Claudette Job
Affiliation:
Centre National de la Recherche Scientifique-Bayer CropScience Joint Laboratory, Unité Mixte de Recherche 5240, Lyon cedex 9, France
Maya Belghazi
Affiliation:
Centre d'Analyses Protéomiques de Marseille, CRN2M-PFRN, CNRS, Aix-Marseille Université, Faculté de médecine Nord, 13015 Marseille, France
Wided Chaibi
Affiliation:
Laboratoire de Biologie et Physiologie Cellulaires Végétales, Département de Biologie, Université de Tunis, Tunisia
Dominique Job
Affiliation:
Centre National de la Recherche Scientifique-Bayer CropScience Joint Laboratory, Unité Mixte de Recherche 5240, Lyon cedex 9, France
*
*Correspondence E-mail: yacoubirafika@yahoo.fr

Abstract

Alfalfa (Medicago sativa L.) yield is severely compromised by soil salinity, especially at the level of seedling establishment. This question was addressed by proteomics to decipher whether specific changes in protein accumulation correlate with germination performance of alfalfa seeds submitted to a salinity stress as obtained by imbibing seeds in the presence of NaCl. This study used alfalfa seeds submitted to an osmopriming invigoration treatment that proved very efficient in counteracting the negative effect of salinity stress on germination performance. Comparative proteomic analyses disclosed 94 proteins commonly characterizing the response of both the untreated control and osmoprimed seeds to the experimental salinity stress. Remarkably, many of them, representing 84 proteins, showed contrasting accumulation patterns when comparing the untreated control and osmoprimed seeds submitted to the same salt stress. Thus numerous changes observed in the proteome of the untreated control seeds imbibed in the presence of salt, and presumably accounting for the loss in seed vigour associated with salinity stress, can be substantially reversed in osmoprimed seeds undergoing this stress. These data therefore provide a biochemical understanding of the increase in seed vigour generally observed with primed seeds.

Type
Research Papers
Copyright
Copyright © Cambridge University Press 2013 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Alam, I., Sharmin, S.A., Kim, K.H., Kim, Y.G., Lee, J.J., Bahk, J.D. and Lee, B.H. (2011) Comparative proteomic approach to identify proteins involved in flooding combined with salinity stress in soybean. Plant Soil 346, 4562.CrossRefGoogle Scholar
Alexander, R., Alamilla, J.M., Salamini, F. and Bartels, D. (1994) A novel embryo-specific barley cDNA clone encodes a protein with homologies to bacterial glucose and ribitol dehydrogenase. Planta 192, 519525.CrossRefGoogle ScholarPubMed
Ambrosone, A., Costa, A., Leone, A. and Grillo, S. (2012) Beyond transcription: RNA-binding proteins as emerging regulators of plant response to environmental constraints. Plant Science 182, 1218.CrossRefGoogle ScholarPubMed
Amooaghaie, R. (2011) The effect of hydro and osmopriming on alfalfa seed germination and antioxidant defenses under salt stress. African Journal of Biotechnology 10, 62686275.Google Scholar
Ashraf, M. and Foolad, M.R. (2005) Pre-sowing seed treatment – a shotgun approach to improve germination, plant growth, and crop yield under saline and non-saline conditions. Advances in Agronomy 68, 223271.CrossRefGoogle Scholar
Bevan, M., Bancroft, I., Bent, E., Love, K., Goodman, H., Dean, C., Bergkamp, R., Dirkse, W., Van Staveren, M., Stiekema, W., et al. (1998) Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana. Nature 391, 485488.Google ScholarPubMed
Bohnert, H.J., Nelson, D.E. and Jensen, R.G. (1995) Adaptations to environmental stresses. The Plant Cell 7, 10991111.CrossRefGoogle ScholarPubMed
Boudet, J., Buitink, J., Hoekstra, F.A., Rogniaux, H., Larré, C., Satour, P. and Leprince, O. (2006) Comparative analysis of the heat stable proteome of radicles of Medicago truncatula seeds during germination identifies late embryogenesis abundant proteins associated with desiccation tolerance. Plant Physiology 140, 14181436.CrossRefGoogle ScholarPubMed
Boughanmi, N., Michonneau, P., Daghfous, D. and Fleurat-Lessard, P. (2005) Adaptation of Medicago sativa cv. Gabès to long-term NaCl stress. Journal of Plant Nutrition and Soil Science 168, 262268.CrossRefGoogle Scholar
Bradford, K.J. (1986) Manipulation of seed water relations via osmotic priming to improve germination under stress conditions. HortScience 21, 11051112.CrossRefGoogle Scholar
Bradford, M. (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principal of protein-dye binding. Analytical Biochemistry 72, 248254.CrossRefGoogle Scholar
Cantero, A., Barthakur, S., Bushart, T.J., Chou, S., Morgan, R.O., Fernandez, M.P., Clark, G.B. and Roux, S.J. (2006) Expression profiling of the Arabidopsis annexin gene family during germination, de-etiolation and abiotic stress. Plant Physiology and Biochemistry 44, 1324.CrossRefGoogle ScholarPubMed
Catusse, J., Meinhard, J., Job, C., Strub, J.M., Fischer, U., Pestsova, E., Westhoff, P., Van Dorsselaer, A. and Job, D. (2011) Proteomics reveals potential biomarkers of seed vigor in sugarbeet. Proteomics 11, 15691580.CrossRefGoogle ScholarPubMed
Cheng, L.B., Gao, X., Li, S.Y., Shi, M.J., Javeed, H., Jing, X.M., Yang, G.X. and He, G.Y. (2010) Proteomic analysis of soybean [Glycine max (L.) Meer.] seeds during imbibition at chilling temperature. Molecular Breeding 26, 117.CrossRefGoogle Scholar
Chu, P., Chen, H., Zhou, Y., Li, Y., Ding, Y., Jiang, L., Tsang, E.W., Wu, K. and Huang, S. (2012) Proteomic and functional analyses of Nelumbo nucifera annexins involved in seed thermotolerance and germination vigor. Planta 235, 12711288.CrossRefGoogle ScholarPubMed
Dure, L. 3rd (1993) A repeating 11-mer amino acid motif and plant desiccation. The Plant Journal 3, 363369.CrossRefGoogle ScholarPubMed
Gallardo, K., Job, C., Groot, S.P.C., Puype, M., Demol, H., Vandekerckhove, J. and Job, D. (2001) Proteomic analysis of Arabidopsis seed germination and priming. Plant Physiology 126, 835848.CrossRefGoogle ScholarPubMed
Gallardo, K., Job, C., Groot, S.P.C., Puype, M., Demol, H., Vandekerckhove, J. and Job, D. (2002) Importance of methionine biosynthesis for Arabidopsis seed germination and seedling growth. Physiologia Plantarum 116, 238247.CrossRefGoogle ScholarPubMed
Hachicha, M. (2007) Les sols salés et leur mise en valeur en Tunisie. Sécheresse 18, 4550.Google Scholar
Hachicha, M., Job, J.O. and Mtimet, A. (1994) Les sols salés et la salinisation en Tunisie. Sols de Tunisie 5, 271341.Google Scholar
Heydecker, W. and Coolbear, P. (1977) Seed treatments for improved performance – survey and attempted prognosis. Seed Science and Technology 5, 353425.Google Scholar
Huh, S.M., Noh, E.K., Kim, H.G., Jeon, B.W., Bae, K., Hu, H.C., Kwak, J.M. and Park, O.K. (2010) Arabidopsis annexins AnnAt1 and AnnAt4 interact with each other and regulate drought and salt stress responses. Plant and Cell Physiology 51, 14991514.CrossRefGoogle ScholarPubMed
Hundertmark, M. and Hincha, D.K. (2008) LEA (Late Embryogenesis Abundant) proteins and their encoding genes in Arabidopsis thaliana. BMC Genomics 9, 118.CrossRefGoogle ScholarPubMed
Job, C., Kersulec, A., Ravasio, L., Chareyre, S., Pépin, R. and Job, D. (1997) The solubilization of the basic subunit of sugarbeet seed 11-S globulin during priming and early germination. Seed Science Research 7, 225243.CrossRefGoogle Scholar
Jörnvall, H., von Bahr-Lindström, H., Jany, K.D., Ulmer, W. and Fröschle, M. (1984) Extended superfamily of short alcohol-polyol-sugar dehydrogenases: structural similarities between glucose and ribitol dehydrogenases. FEBS Letters 165, 190196.CrossRefGoogle ScholarPubMed
Kalemba, E.M. and Pukacka, S. (2008) Changes in late embryogenesis abundant proteins and a small heat shock protein during storage of beech (Fagus sylvatica L.) seeds. Environmental and Experimental Botany 63, 274280.CrossRefGoogle Scholar
Lee, S., Lee, E.J., Yang, E.J., Lee, J.E., Park, A.R., Song, W.H. and Park, O.K. (2004) Proteomic identification of annexins, calcium-dependent membrane binding proteins that mediate osmotic stress and abscisic acid signal transduction in Arabidopsis. The Plant Cell 16, 13781391.CrossRefGoogle ScholarPubMed
Liu, K.L., Xu, S., Xuan, W., Ling, T.F., Cao, Z., Huang, B.K., Sun, Y.G., Fang, L., Liu, Z.Y., Zhao, N. and Shen, W.B. (2007) Carbon monoxide counteracts the inhibition of seed germination and alleviates oxidative damage caused by salt stress in Oryza sativa. Plant Science 172, 544555.CrossRefGoogle Scholar
Liu, Y., Xu, S., Ling, T., Xu, L. and Shen, W. (2010) Heme oxygenase/carbon monoxide system participates in regulating wheat seed germination under osmotic stress involving the nitric oxide pathway. Journal of Plant Physiology 167, 13711379.CrossRefGoogle ScholarPubMed
Lorkovic, Z.J. (2009) Role of plant RNA-binding proteins in development, stress response and genome organization. Trends in Plant Science 14, 229236.CrossRefGoogle ScholarPubMed
Maitra, N. and Cushman, J.C. (1994) Isolation and characterization of a drought-induced soybean cDNA encoding a D95 family late-embryogenesis-abundant protein. Plant Physiology 106, 805806.CrossRefGoogle ScholarPubMed
Noctor, G. and Foyer, C.H. (1998) Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49, 249279.CrossRefGoogle ScholarPubMed
Otterbein, L.E., Soares, M.P., Yamashita, K. and Bach, F.H. (2003) Heme oxygenase-1: unleashing the protective properties of heme. Trends in Immunology 24, 449455.CrossRefGoogle ScholarPubMed
Peel, M.D., Waldron, B.L., Jensen, K.B., Chatterton, N.J., Horton, H. and Dudley, L.M. (2004) Screening for salinity tolerance in alfalfa: a repeatable method. Crop Science 44, 20492053.CrossRefGoogle Scholar
Rajjou, L., Lovigny, Y., Groot, S.P.C., Belghazi, M., Job, C. and Job, D. (2008) Proteome-wide characterization of seed aging in Arabidopsis: a comparison between artificial and natural aging protocols. Plant Physiology 148, 620641.CrossRefGoogle ScholarPubMed
Rajjou, L., Duval, M., Gallardo, K., Catusse, J., Bally, J., Job, C. and Job, D. (2012) Seed germination and vigor. Annual Review of Plant Biology 63, 507533.CrossRefGoogle ScholarPubMed
Ravanel, S., Gakière, B., Job, D. and Douce, R. (1998) The specific features of methionine biosynthesis and metabolism in plants. Proceedings of the National Academy of Sciences, USA 95, 78057812.CrossRefGoogle ScholarPubMed
Roxas, V.P., Lodhi, S.A., Garrett, D.K., Mahan, J.R. and Allen, R.D. (2000) Stress tolerance in transgenic tobacco seedlings that overexpress glutathione S-transferase/glutathione peroxidase. Plant and Cell Physiology 41, 12291234.CrossRefGoogle ScholarPubMed
Soeda, Y., Konings, M.C.J.M., Vorst, O., van Houwelingen, A.M.M.L., Stoopen, G.M., Maliepaard, C.A., Kodde, J., Bino, R.J., Groot, S.P.C. and van der Geest, A.H.M. (2005) Gene expression programs during Brassica oleracea seed maturation, osmopriming, and germination are indicators of progression of the germination process and the stress tolerance level. Plant Physiology 137, 354368.CrossRefGoogle ScholarPubMed
Waanders, L.F., Chwalek, K., Monetti, M., Kumar, C., Lammert, E. and Mann, M. (2009) Quantitative proteomic analysis of single pancreatic islets. Proceedings of the National Academy of Sciences, USA 106, 1890218907.CrossRefGoogle ScholarPubMed
Wehmeyer, N. and Vierling, E. (2000) The expression of small heat shock proteins in seeds responds to discrete developmental signals and suggests a general protective role in desiccation tolerance. Plant Physiology 122, 10991108.CrossRefGoogle ScholarPubMed
Witzel, K., Weidner, A., Surabhi, G.K., Varshney, R.K., Kunze, G., Buck-Sorlin, G.H., Börner, A. and Mock, H.P. (2010) Comparative analysis of the grain proteome fraction in barley genotypes with contrasting salinity tolerance during germination. Plant, Cell and Environment 33, 211222.CrossRefGoogle ScholarPubMed
Xu, D., Duan, X., Wang, B., Hong, B., Ho, T.H.D. and Wu, R. (1996) Expression of a late embryogenesis abundant protein gene, HVAI, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiology 110, 249257.CrossRefGoogle Scholar
Xu, S., Lou, T.L., Zhao, N., Gao, Y., Dong, L.H., Jiang, D.J., Shen, W.B., Huang, L.Q. and Wang, R. (2011) Presoaking with hemin improves salinity tolerance during wheat seed germination. Acta Physiologiae Plantarum 33, 11731183.CrossRefGoogle Scholar
Xu, X.Y., Fan, R., Zheng, R., Li, C.M. and Yu, D.Y. (2011) Proteomic analysis of seed germination under salt stress in soybeans. Journal of Zhejiang University, Science B 12, 507517.CrossRefGoogle ScholarPubMed
Yacoubi, R., Job, C., Belghazi, M., Chaibi, W. and Job, D. (2011) Toward characterizing seed vigor in alfalfa through proteomic analysis of germination and priming. Journal of Proteome Research 10, 38913903.CrossRefGoogle ScholarPubMed
Supplementary material: File

Yacoubi et al. supplementary material

Supplementary material

Download Yacoubi et al. supplementary material(File)
File 487.4 KB
Supplementary material: File

Yacoubi et al. supplementary material

Supplementary material

Download Yacoubi et al. supplementary material(File)
File 83.7 KB
Supplementary material: File

Yacoubi et al. supplementary material

Supplementary material

Download Yacoubi et al. supplementary material(File)
File 84.2 KB