Skip to main content Accessibility help
×
Home
Hostname: page-component-55b6f6c457-s8qdg Total loading time: 0.209 Render date: 2021-09-24T21:08:48.907Z Has data issue: true Feature Flags: { "shouldUseShareProductTool": true, "shouldUseHypothesis": true, "isUnsiloEnabled": true, "metricsAbstractViews": false, "figures": true, "newCiteModal": false, "newCitedByModal": true, "newEcommerce": true, "newUsageEvents": true }
RNA RNA

Article contents

An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association

Published online by Cambridge University Press:  29 June 2001

STEVEN WORMSLEY
Affiliation:
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA
DMITRY A. SAMARSKY
Affiliation:
Program in Molecular Medicine, University of Massachusetts Medical Center, Worcester, Massachusetts 01605, USA
MAURILLE J. FOURNIER
Affiliation:
Departments of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, Massachusetts 01605, USA
SUSAN J. BASERGA
Affiliation:
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA Department of Genetics, Yale University School of Medicine, New Haven, Connecticut 06520-8040, USA
Get access

Abstract

The U3 small nucleolar ribonucleoprotein (snoRNP) is composed of a small nucleolar RNA (snoRNA) and at least 10 proteins. The U3 snoRNA base pairs with the pre-rRNA to carry out the A0, A1, and A2 processing reactions that lead to the release of the 18S rRNA from the nascent pre-rRNA transcript. The yeast U3 snoRNA can be divided into a short 5′ domain (nt 1–39) and a larger 3′ domain (73 to the 3′ end) separated by a stretch of nucleotides called the hinge region (nt 40–72). The sequences required for pre-rRNA base pairing are found in the 5′ domain and hinge region whereas the 3′ domain is largely covered with proteins. Mpp10p, one of the protein components unique to the U3 snoRNP, plays a role in processing at the A1 and A2 sites. Because of its critical role in U3 snoRNP function, we determined which sequences in the U3 snoRNA are required for Mpp10p association. Unlike fibrillarin and all the previous U3 snoRNP components studied in this manner, sequences in the 3′ domain are not sufficient for Mpp10p association. Instead, a conserved sequence element in the U3 snoRNA hinge region is required, placing Mpp10p near the 5′ domain that carries out the pre-rRNA base-pairing interactions in the functional center of the U3 snoRNP.

Type
Research Article
Copyright
2001 RNA Society

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Send article to Kindle

To send this article to your Kindle, first ensure no-reply@cambridge.org is added to your Approved Personal Document E-mail List under your Personal Document Settings on the Manage Your Content and Devices page of your Amazon account. Then enter the ‘name’ part of your Kindle email address below. Find out more about sending to your Kindle. Find out more about sending to your Kindle.

Note you can select to send to either the @free.kindle.com or @kindle.com variations. ‘@free.kindle.com’ emails are free but can only be sent to your device when it is connected to wi-fi. ‘@kindle.com’ emails can be delivered even when you are not connected to wi-fi, but note that service fees apply.

Find out more about the Kindle Personal Document Service.

An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association
Available formats
×

Send article to Dropbox

To send this article to your Dropbox account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Dropbox.

An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association
Available formats
×

Send article to Google Drive

To send this article to your Google Drive account, please select one or more formats and confirm that you agree to abide by our usage policies. If this is the first time you use this feature, you will be asked to authorise Cambridge Core to connect with your <service> account. Find out more about sending content to Google Drive.

An unexpected, conserved element of the U3 snoRNA is required for Mpp10p association
Available formats
×
×

Reply to: Submit a response

Please enter your response.

Your details

Please enter a valid email address.

Conflicting interests

Do you have any conflicting interests? *