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Zone-plate X-ray microscopy

Published online by Cambridge University Press:  17 March 2009

G. Schmahl ,
D. Rudolph ,
B. Niemann  and
O. Christ
G. Schmahl
Affiliation:
University of Göingen, Universitäts-Sternwarte, Geismarlandstrasse 11, 34 Göttingen, W. Germany
D. Rudolph
Affiliation:
University of Göingen, Universitäts-Sternwarte, Geismarlandstrasse 11, 34 Göttingen, W. Germany
B. Niemann
Affiliation:
University of Göingen, Universitäts-Sternwarte, Geismarlandstrasse 11, 34 Göttingen, W. Germany
O. Christ
Affiliation:
University of Göingen, Universitäts-Sternwarte, Geismarlandstrasse 11, 34 Göttingen, W. Germany
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Extract

The scope of this article is to describe a transmission X-ray microscope, possible biological applications of soft X-ray microscopy and preliminary results.

For soft X-ray microscopy of biological specimens the wavelength range of 1–10 nm is best suited. Microscopy in this wavelength range requires intense X-ray sources as well as high-resolution X-ray lenses. Intense X-radiation is provided by the synchrotron radiation of electron and positron storage rings. Suited X-ray lenses are zone plates.

A theoretical treatment of the contrast mechanism and the radiation damage as well as first experiments yield the following results. Firstly, relatively thick (1–10 μm) biological specimens can be investigated. This means that unsectioned dried and even wet cells and cell organelles in a natural state can be examined. Second, it will be possible to resolve cellular aggregates in live cells with a resolution in the region of ≤10nm.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 13 , Issue 3 , August 1980 , pp. 297 - 315
Copyright
Copyright © Cambridge University Press 1980

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References

REFERENCES

Engström, A. (1946). Quantitative micro-and histochemical elementary analysis by Roentgen absorption spectrography. Acta radiol., Suppl. 63, 1–106.Google Scholar
Flanders, D. C., Shaver, D. C., Hawryluk, A. M.& Smith, H. I. (1980). Progress in the fabrication of artificial microstructures for X-ray imaging and spectroscopy. In Ultrasoft X-ray microscopy: Its Application to Biological and Physical Sciences. Ann. N.Y. Acad. Sci. 342, 203–212.Google Scholar
Kirz, J. (1980). Specimen damage considerations in biological microprobe analysis. In Scanning Electron Microscopy. Chicago: SEM Inc., AMF O'Hare.Google Scholar
Niemann, B., Rudolph, D.& Schmahl, G. (1974). Soft X-ray imaging zone plates with large zone numbers for microscopic and spectroscopic applications. Opt. Commun. 12, 160–163.Google Scholar
Niemann, B., Rudolph, D.& Schmahl, G. (1976). X-ray microscopy with synchrotron radiation. Appl. Optics 15, 1883.CrossRefGoogle ScholarPubMed
Parsons, D. F., Matricardi, V. R., Moretz, R. C.& Turner, J. N. (1974). Electron microscopy and diffraction of wet unstained and unfixed biological objects. Adv. biol. med. Phys. 15, 161–270.Google Scholar
Rosser, R. (1980). Proposal for a soft X-ray microscope at SSR Daresbury. Report, Blackett Laboratory, Imperial College, London, SW72BZ.Google Scholar
Rudolph, D.& Schmahl, G. (1980). High power zone plates for a soft X-ray microscope. In Ultrasoft X-ray microscopy: Its application to biological and physical sciences. Ann. N. Y. Acad. Sci. 342, 94–104.Google Scholar
Sayre, D., Kirz, J., Feder, R., Kim, D. M.& Spiller, E. (1977). Transmission microscopy of unmodified biological materials: comparative radiation dosages with electrons and ultrasoft X-ray photons. Ultra- microscopy 2, 337–349.Google ScholarPubMed
Schmahl, G., Rudolph, D.& Niemann, B. (1980). Imaging and scanning soft X-ray microscopy with zone plates. Proc. Int. Symp. ‘Scanned Image Microscopy’. The Royal Society, London.Google Scholar
Schmahl, G., Rudolph, D., Niemann, B.& Christ, O. (1980). X-ray microscopy of biological specimens with a zone-plate microscope. In Ultrasoft X-ray Microscopy: Its Application to Biological and Physical Sciences. Ann. N.Y. Acad. Sci. 342, 368–386.Google Scholar