This inquiry was commenced in Shanghai at the end of 1884, soon after Koch had published some of his results, and as he was then almost the only worker in the bacteriological field of whom it could be said that he had not published anything, subsequently upset, attention was directed at once to the“comma bacillus.”

Slightly alkaline peptonised meat jelly on plates was used for purposes of cultivation, this being kept at a temperature of 60° to 70° Fahr. by means of an ice chest in hot weather.

The investigators worked independently, but the majority of the cases of cholera under observation were investigated by both.