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The potential of immortalised mammalian cells for the advancement of drug discovery

Published online by Cambridge University Press:  05 December 2011

Caroline MacDonald
Caroline MacDonald
Affiliation:
Department of Immunology, University of Strathclyde, Todd Centre, 31 Taylor Street, Glasgow G4 0NR, U.K.
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Synopsis:

Oncogenes can be introduced into cells which have a limited lifespan, and immortalised cell lines isolated as a result. These cell lines often retain the differentiated, tissue-specific characteristics found in the original cells and, as such, provide an important model for pharmacological studies. The techniques used to develop cell lines from rabbit kidney, mouse macrophages and rat liver are described. A preliminary characterisation of all three types of cells has been carried out, and in each case the immortalised lines described have many of the properties of the original tissue type.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 99 , Issue 1-2: The Advancement of Drug Discovery , 1992 , pp. 47 - 54
Copyright
Copyright © Royal Society of Edinburgh 1992

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References

Brzeski, H., Chambers, M., MacDonald, C. & Stimson, W. H. 1985. The immortalisation of human lymphocytes by spheroplast fusion. Developments of Biological Standardisation 60, 105–9.Google Scholar
Cuzin, F. 1984. The polyoma virus oncogenes: coordinated functions of three distinct proteins in the transformation of rodent cells in culture. Biochimica, Biophysica Acta 781, 193–204.CrossRefGoogle ScholarPubMed
Eveloff, J., Haase, W. & Kinne, R. 1980. Separation of renal medullary cells: isolation of cells from the thick ascending limb of Henle's loop. Journal of Cell Biology 87, 672–81.CrossRefGoogle ScholarPubMed
Gorman, C. 1985. High efficiency gene transfer into mammalian cells. In DNA cloning, pp. 143–90; vol. II, ed Glover, D. M., Oxford: IRL Press.Google Scholar
Gorman, C., Padmanabhan, R. & Howard, B. 1983. High efficiency DNA-mediated transformation of primate cells. Science 221, 551–3.CrossRefGoogle ScholarPubMed
Habig, W. H. & Jakoby, W. B. 1981. Assays for differentiation of glutathione S transferases. Methods in Enzymology 77, 398–405.CrossRefGoogle ScholarPubMed
Isom, H. C. & Georgoff, I. 1984. Quantitative assay for albumin-producing liver cells after simian virus 40 transformation of rat hepatocytes maintained in chemically defined medium. Proceedings of the National Academy of Science, USA 81, 6378–82.CrossRefGoogle ScholarPubMed
Kreuzburg-Duffy, U. & MacDonald, C. 1991. Establishment of immortalised cell lines from mouse peritoneal macrophages following transformation with SV40 early region DNA deleted at the origin of replication. Immunology 72, 368–72.Google Scholar
Land, H., Parada, L. F. & Weinberg, R. A. 1983. Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating oncogenes. Nature (Lond.) 304, 596–602.CrossRefGoogle ScholarPubMed
MacDonald, C. 1990. Development of new cell lines for animal cell biotechnology. Critical Reviews in Biotechnology 10, 155–78.CrossRefGoogle ScholarPubMed
MacDonald, C. 1991b. Genetic engineering of animal cells. In Mammalian cell biotechnology, a practical approach, pp. 57–83, ed. Butler, M. Oxford: IRL Press.CrossRefGoogle Scholar
MacDonald, C., Watts, P., Stuart, B., Kreuzburg-Duffy, U., Scott, D. M. & Kinne, R. K. H. 1991a. Studies on the phenotype and karyotype of immortalized rabbit kidney epithelial cell lines. Experimental Cell Research 195, 458–61.CrossRefGoogle ScholarPubMed
Mulligan, R. C. & Berg, P. 1980. Expression of a bacterial gene in mammalian cells. Science 209, 1422–7.CrossRefGoogle ScholarPubMed
Nairn, A., Willett, B., Grant, M. H., Scott, A. & MacDonald, C. 1990. Maintenance of differentiated function in cultured rat hepatocytes immortalised by transfection with viral DNA. Biochemical Society Transactions 18, 1201–02.CrossRefGoogle Scholar
Rassoulzadegan, M., Cowie, A., Carr, A., Glaichenhaus, N., Kamen, R. & Cuzin, F. 1982. The roles of individual polyoma virus early proteins in oncogenic transformation. Nature (Lond.) 300, 713–18.CrossRefGoogle ScholarPubMed
Ruley, H. E. 1983. Adenovirus early region 1A enables viral and cellular transforming genes to transform primary cells in culture. Nature (Lond.) 304, 602–06.CrossRefGoogle ScholarPubMed
Scott, D. M., MacDonald, C., Brzeksi, H. & Kinne, R. 1986. Maintenance of expression of differentiated function of kidney cells following transformation by SV40 early region DNA. Experimental Cell Research 166, 391–8.CrossRefGoogle ScholarPubMed
Scott, D. M., Brzeksi, H., Kinne, R. & MacDonald, C. 1989. Transfection of differentiated primary kidney cell cultures by early region SV40 virus DNA. In Advances in animal cell biology and technology for bioprocesses, pp. 198–200, eds, Spier, R. E., Griffiths, J. B., Stephenne, J. & Crooy, P. London: Butterworths.Google Scholar
Southern, P. J. & Berg, P. 1982. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under the control of the SV40 early region promoter. Journal of Molecular and Applied Genetics 1, 327–41.Google ScholarPubMed
Spandidos, D. A. & Wilkie, N. M. 1984. Malignant transformation of early passage rodent cells by a single mutated human oncogene. Nature (Lond.) 310, 469–75.CrossRefGoogle ScholarPubMed
Williams, G. M., Bermudez, E. & Scaramuzzino, L. A. 1977. Rat hepatocyte primary cell cultures. III Improved dissociation and attachment techniques and the enhancement of survival by culture medium. In Vitro 13, 809–17.CrossRefGoogle ScholarPubMed