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Antioxidants and free radical oxidation as regulators of ATP biosynthesis in chloroplasts

Published online by Cambridge University Press:  05 December 2011

N. V. Budagovskaya
N. V. Budagovskaya
Affiliation:
Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya 35, 127276 Moscow, Russia
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Synopsis

A study has been made of the effects of natural antioxidants (cysteine and ascorbate), commonly used in media for isolation of organelles and in reaction mixtures, on light-induced proton uptake and photophosphorylation in chloroplasts of pea and maize. These plants were chosen because of their different levels of resistance to unfavourable environmental conditions. Conditions for studying chloroplasts in different functional states were modelled and it was shown that the antioxidants exerted a regulatory effect on light-induced proton uptake and ATP formation. The direction of the response was dependent on the initial state of the chloroplasts and was correlated with the concentrations of free and total sulfhydryl groups. A scheme for regulation of energy transformation in chloroplasts, including antioxidants and free radical oxidation, is proposed.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 237 - 241
Copyright
Copyright © Royal Society of Edinburgh 1994

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References

Arnon, D. 1949. Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiology 24, 1–15.CrossRefGoogle ScholarPubMed
Avron, M. A. 1963. A coupling factor in photophosphorylation. Biochimica et Biophysica Acta 77, 699–705.CrossRefGoogle Scholar
Baslavskaya, S. S. & Trubezkova, O. M. 1964. Practical works on plant physiology. Moscow: Moscow State University.Google Scholar
Habeeb, A. F. S. A. 1972. Reaction of protein sulfhydryl groups with Ellman's reagent. In Hirs, C. H. W. & Timasheff, S. N. (Eds) Methods in enzymology, Vol. XXV, pp. 457–64. New York & London: Academic Press.Google Scholar
Lowry, O., Rosenbrough, N., Farr, A. & Randall, R. 1951. Protein measurement with Folin phenol reagent. Journal of Biological Chemistry 193, 265–75.CrossRefGoogle ScholarPubMed
Nishimura, M., Ito, T. & Chance, B. 1962. Studies on bacterial photophosphorylation. III. A sensitive and rapid method of determinating photophosphorylation. Biochimica et Biophysica Acta 59, 623–7.Google Scholar
Thompson, J. E., Legge, R. L. & Barger, R. F. 1987. The role of free radicals in senescence and wounding. New Phytologist 105, 317–344.CrossRefGoogle ScholarPubMed
Whatley, F. R. & Arnon, D. J. 1963. Photosynthetic phosphorylation in plants. In Hirs, C. H. W. & Timasheff, S. N. (Eds) Methods in Enzymology, Vol. VI, pp. 308–13. New York & London: Academic Press.Google Scholar