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Repeatability of in vitro digestibility assays of forages when using fresh, frozen or freeze-dried cow faeces instead of sheep rumen liquor as sources of micro-organisms

Published online by Cambridge University Press:  20 November 2017

S Akhter ,
E Owen ,
M K Theodorou ,
S L Tembo  and
E R Deaville
S Akhter
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading, Berks RG6 2AT
E Owen
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading, Berks RG6 2AT
M K Theodorou
Affiliation:
BBSRC Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Dyfed SY23 3EB
S L Tembo
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading, Berks RG6 2AT
E R Deaville
Affiliation:
Feed Evaluation Unit, ADAS Dairy Research Centre, Drayton, Stratford-upon-Avon, Warwicks CV37 9RQ
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Extract

Previous studies (El Shaer, Omed and Axford, 1987; Akhter, Owen, Fall, O'Donovan and Theodorou, 1994) with the two-stage in vitro procedure of Tilley and Terry (1963) have shown a high correlation between digestibilities of forages as determined using either sheep rumen liquor, sheep faeces or cow faeces as the microbial inoculum. In the first study of the of the present investigation one objective was to examine the repeatability of these digestibility measurements when made on different occasions. A second objective was to assess whether the correlations between faecal and rumen fluid based inocula could be improved if microorganisms were obtained from pairs rather than individual animals. The objective in the second study using forages of known in vivo digestibility, was to investigate the effect of freezing or freeze-drying of faeces on the repeatability of digestibilities of forages determined in vitro using micro-organisms from cow faeces.

Type
Techniques and Methods
Information
Proceedings of the British Society of Animal Science , Volume 1995 , March 1995 , pp. 110
Copyright
Copyright © The British Society of Animal Science 1995

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References

Akhter, S., Owen, E., Fall, A., O'Donovan, F. and Theodorou, M.K. 1994, Use of fresh or frozen faeces instead of sheep rumen liquor to provide micro-organisms for in vitro digestibility assays for forages. Animal Production 58: 452.Google Scholar
EL Shaer, H.M., Omed, H.M., Chamberlain, A.G. and Axford, R.F.E. 1987. Use of faecal organisms from sheep for the in vitro determination of digestibility. Journal of Agricultural Science, Cambridge 109: 257–259.10.1017/S0021859600080667CrossRefGoogle Scholar
Tilley, J.M.A. and Terry, R.A. 1963. A two stage technique for in vitro digestion of forage crops. Journal of the British Grassland Society 18:104–111.10.1111/j.1365-2494.1963.tb00335.xCrossRefGoogle Scholar