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A low tech in vitro procedure using faecal liquor for the estimation of digestibility of Forages

Published online by Cambridge University Press:  05 November 2021

H.M. Omed ,
A. Faza ,
R.F.E. Axford ,
I. Ap Dewi  and
D.I. Givens
H.M. Omed
Affiliation:
School of Agricultural & Forest sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK
A. Faza
Affiliation:
School of Agricultural & Forest sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK
R.F.E. Axford
Affiliation:
School of Agricultural & Forest sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK
I. Ap Dewi
Affiliation:
School of Agricultural & Forest sciences, University of Wales, Bangor, Gwynedd, LL57 2UW, UK
D.I. Givens
Affiliation:
ADAS Feed Evaluation & Nutritional Sciences, Stratford upon Avon, CV37 9RQ
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Extract

Two-stage techniques Tilley & Terry (1963) for the estimation of digestibility have 3 main disadvantages for use in developing countries with limited laboratory facilities. Carbon dioxide is needed to saturate the buffers; a centrifuge is necessary to separate residues from solubilised materials; and the methods uses pepsin as a proteolytic agent. The aim of the present study was to eliminate these three requirements for the faecal liquor method ( Omed et al., 1989), by replacing bicarbonate with phosphate buffer (Marten & Barnes, 1980), pepsin with biological washing liquid, and centrifugation with sedimentation.

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Programme
Information
Proceedings of the British Society of Animal Science , Volume 1998 , 1998 , pp. 59
Copyright
Copyright © The British Society of Animal Science 1998

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References

Marten, G.C. and Barnes, R.F. (1980). Prediction of energy digestibility of forages. In Standarization of analytical methodology for feeds. Eds. Pigden, W. & Graham, M., International development centre, Ottawa, Canada Google Scholar
Omed, H.M., Axford, R.F.E., Chamberlain, A.G. & Givens, D.I. (1989). a comparison of three laboratory techniques for the estimation of the digestibility of feedstuffs for ruminants. Journal of agricultural Science 13, 35–39.10.1017/S0021859600084586CrossRefGoogle Scholar
Tilley, J.M. and Terry, R.A. (1963). A two-stage technique for the in-vitro digestion of forage crops. Journal of British Grassland Society, 18, 104–111.10.1111/j.1365-2494.1963.tb00335.xCrossRefGoogle Scholar