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The influence of biotin on viability, pyruvate carboxylase activity and the response to malate concentration in vitro by isolated secondary hair follicles of the Angora goat

Published online by Cambridge University Press:  20 November 2017

A.M. Tahmasbi ,
H. Galbraith  and
J.R. Scaife
A.M. Tahmasbi
Affiliation:
Department of Agriculture, Aberdeen University, 581 King Street, Aberdeen, ScotlandAB24 5UA, UK
H. Galbraith
Affiliation:
Department of Agriculture, Aberdeen University, 581 King Street, Aberdeen, ScotlandAB24 5UA, UK
J.R. Scaife
Affiliation:
Department of Agriculture, Aberdeen University, 581 King Street, Aberdeen, ScotlandAB24 5UA, UK
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Extract

Biotin is a prosthetic group for a number of enzymes involved in carboxylation reactions. Biotin deficiency has been associated with poor growth of integumental tissues by currently unknown mechanisms. Pyruvate carboxylase is a biotin-dependent enzyme which has an important anaplerotic role in intermediary metabolism, catalysing the formation of oxaloacetate from pyruvate and bicarbonate. It thus provides oxaloacetate for gluconeogenesis and replenishes Krebs cycle intermediates. Biotin deficiency may reduce pyruvate carboxylase activity and so oxaloacetate production. Malate is a Krebs cycle intermediate which can convert to oxaloacetate and reduce demand for oxaloacetate generated by pyruvate carboxylase. The aims of the study were to determine (a) the effect of biotin supplementation of the medium on hair follicle viability and pyruvate carboxylase activity and (b) the response to supplementation of the culture medium with malate.

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Programme
Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 158
Copyright
Copyright © The British Society of Animal Science 1999

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References

Ibraheem, M., Galbraith, H., Scaife, J. R. and Ewen, S. 1993. Growth and viability of secondary hair follicles of the Angora goat cultured in vitro. Journal of Anatomy 182, 213–238.Google ScholarPubMed
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