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Glucose metabolism of in vitro-produced bovine embryos in cell-free and co-culture systems

Published online by Cambridge University Press:  20 November 2017

N.M. Orsi ,
J.B. Reischl  and
H.J. Leese
N.M. Orsi
Affiliation:
Department of Biology, University of York, PO Box 373, Heslington YO10 5YW, UK
J.B. Reischl
Affiliation:
Gynaekologische und Ambulatorische Tierklinik, LMU Muenchen, Koenigestrasse 12, 80539 Muenchen, Germany
H.J. Leese
Affiliation:
Department of Biology, University of York, PO Box 373, Heslington YO10 5YW, UK
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Extract

In vitro-produced (IVP) bovine embryos are used in a wide range of biotechnologies but develop less well than their in vivo counterparts and can give rise to foetal/neonatal anomalies after embryo transfer. The quality of bovine IVP embryos and the systems in which they are produced are traditionally assessed in terms of morphological and developmental criteria; namely, embryo grade and blastocyst formation rate. Lane and Gardner (1996) showed that mouse embryos selected for transfer on the basis of a low glycolytic activity (conversion of glucose to lactate), measured non-invasively, were 4 times more likely to implant than those selected randomly. Comparable data are not available for bovine embryos. The aim of this study was to assess linear glycolytic index of cattle blastocysts in vitro as a marker of viability. We have measured glucose consumption and lactate production by individual bovine IVP embryos grown in cell-free conditions and in a novel co-culture system (Orsi et al., 2000) involving confluent bovine oviduct epithelial cell monolayers on permeable supports. This preparation allows the epithelial cells to be fed by a nutritionally-rich medium via the physiological, basal, route, while the apical medium, containing the embryos, is more dilute, mimicking oviduct fluid.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2001 , 2001 , pp. 216
Copyright
Copyright © The British Society of Animal Science 2001

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References

Gardner, D.K. and Leese, H.J. 2000. Assessment of embryo metabolism and viability. In: Trounson, AO & Gardner, DK (eds) Handbook of In Vitro Fertilization (2nd ed), CRC Press, 347–371 Google Scholar
Lane, M. and Gardner, D.K. 1996. Selection of viable mouse blastocysts prior to transfer using a metabolic criterion. Human Reproduction 11: 1975–1978.CrossRefGoogle ScholarPubMed
Orsi, N.M., Leese, H.J. and Reischl, J.B. 2000. A novel somatic cell co-culture system for in vitro-produced bovine embryos. Journal of Reproduction and Fertility Abstract Series 25: 75–76.Google Scholar
Thompson, J.G., Partridge, R.J., Houghton, F.D., Cox, C.I. and Leese, H.J. 1996. Oxygen uptake and carbohydrate metabolism by in vitro derived bovine embryos. Journal of Reproduction and Fertility, 106: 299–306.Google Scholar