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Expression of igf-i and gh-receptor (ghr) mrna in liver and skeletal muscle of different pig breeds

Published online by Cambridge University Press:  20 November 2017

J.M. Brameld ,
J.L. Atkinson ,
T.J. Budd ,
J.C. Saunders ,
A.M. Salter ,
R.S. Gilmour  and
P.J. Buttery
J.M. Brameld
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
J.L. Atkinson
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
T.J. Budd
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
J.C. Saunders
Affiliation:
Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge, CB2 4AT
A.M. Salter
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
R.S. Gilmour
Affiliation:
Department of Cellular Physiology, The Babraham Institute, Babraham, Cambridge, CB2 4AT
P.J. Buttery
Affiliation:
University of Nottingham, Department of Applied Biochemistry and Food Science, Sutton Bonington Campus, Loughborough, LE12 5RD
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Extract

Previous studies of the porcine GH-IGF axis have demonstrated age related changes in liver, but not LD muscle, in expression of both GH-receptor (GHR) and IGF-I genes (Brameld et al, 1993; 1995), and also effects of energy availability on the expression of IGF-I in liver, but not in LD muscle (Weller et al, 1994). The work described here was carried out to study the expression of IGF-I and GHR mRNA in liver and skeletal muscle from different breeds of pig. The probes used were as described previously (Brameld et al, 1993; 1995; Weller et al, 1993; 1994), and were designed to monitor promoter usage for IGF-I expression, with the two promoters giving rise to class 1 and 2 transcripts, and also expression of the extra-cellular domain of the GH-receptor.

Type
Posters for Theatre Session
Information
Proceedings of the British Society of Animal Science , Volume 1995 , March 1995 , pp. 183
Copyright
Copyright © The British Society of Animal Science 1995

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References

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