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Effects of oil source, feed and pH on biohydrogenation of linoleic acid and production of trans-10, cis-12 conjugated linoleic acid (CLA) in vitro

Published online by Cambridge University Press:  23 November 2017

W. M. P. B Weerasinghe ,
J. A. Huntington ,
R. G. Wilkinson  and
L. A. Sinclair
W. M. P. B Weerasinghe*
Affiliation:
Harper Adams University College, Newport, Shropshire, TF10 8NB, United Kingdom
J. A. Huntington
Affiliation:
Harper Adams University College, Newport, Shropshire, TF10 8NB, United Kingdom
R. G. Wilkinson
Affiliation:
Harper Adams University College, Newport, Shropshire, TF10 8NB, United Kingdom
L. A. Sinclair
Affiliation:
Harper Adams University College, Newport, Shropshire, TF10 8NB, United Kingdom
*
Email:tweerasinghe@harper-adams.ac.uk
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Extract

Trans-10, cis-12 CLA is produced as an intermediary during the biohydrogenation of linoleic acid (C18:2 n-6) in the rumen and has been shown to be a potent inhibitor of milk fat synthesis in ruminants. The production of trans-10, cis-12 CLA in the rumen is affected by dietary concentrate: forage ratio (Kucuk et al., 2001), rumen pH and the amount and source of linoleic acid in the diet. However, the interaction between oil source, carbohydrate source and pH on the production of trans-10, cis-12 CLA is unclear (Beam et al., 2000). The objectives of the current study were to determine the effects of oil source, carbohydrate source and pH on the biohydrogenation of linoleic acid and production of trans-10, cis-12 CLA in vitro.

Type
Theatre Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2008 , 2008 , pp. 27
Copyright
Copyright © The British Society of Animal Science 2008

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References

Beam, T.M., Jenkins, T.C., Moate, P.J., Kohn, R.A. and Palmquist, D. L. 2000. Effects of amount and source of fat on the rates of lipolysis and biohydrogenation of fatty acids in ruminal contents. Journal of Dairy Science 83: 2564–2573.Google Scholar
Kucuk, O., Hess, B.W., Ludden, P.A. and Rule, D.C. 2001. Effect of forage: concentrate ratio on ruminal digestion and duodenal flow of fatty acids in ewes. Journal of Animal Science 79: 2233–2240.CrossRefGoogle ScholarPubMed