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Development of porcine embryos cultured individually in vitro using NCSU-23 and modified Whitten's media

Published online by Cambridge University Press:  05 November 2021

T.G. McEvoy
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
M.E. Staines
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
G.D. Coull
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
M.E. King
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
M. Slessor
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
H. Fraser
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
H. Fraser
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
S.A. Edwards
Affiliation:
Scottish Agricultural College, Craibstone Estate, Bucksburn, Aberdeen AB21 9YA, UK
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Extract

Individual culture of porcine embryos facilitates precise evaluation of developmental progress during crucial stages of preimplantation growth in vitro. This approach was applied to embryos, collected at different stages of development, from young pre-pubertal gilts induced to ovulate following gonadotrophin treatment. The present study also examined embryo development in 2 media produced for porcine egg culture, NCSU-23 (Petters and Wells, 1993) and modified Whitten's Medium (mWM; Beckmann and Day, 1993).

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Copyright
Copyright © British Society of Animal Science 1997

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References

Petters, RM and Wells, KD (1993). Journal of Reproduction and Fertility, Supplement 48: 6173.Google Scholar
Beckmann, LS and Day, BN (1993). Theriogenology 39: 611622.CrossRefGoogle Scholar