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Comparison of sheep rumen liquor and Rusitec fluid as inoculum for determining the in vitro digestibility of hays

Published online by Cambridge University Press:  20 November 2017

M.L. Tejido ,
M.J. Ranilla  and
M.D. Carro
M.L. Tejido
Affiliation:
Departamento de Producción Animal I. Universidad de León. 24071 León. Spain
M.J. Ranilla
Affiliation:
Departamento de Producción Animal I. Universidad de León. 24071 León. Spain
M.D. Carro
Affiliation:
Departamento de Producción Animal I. Universidad de León. 24071 León. Spain
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Extract

One practical disadvantage of some in vitro methods used to estimate the in vivo digestibility of forages is the need for fistulated donor ruminants to provide the rumen liquor. These are subject to restrictive legislation in many countries and are costly to prepare and maintain. The aim of this study was to investigate whether rumen liquor in the in vitro digestibility technique of Van Soest et al. (1966) could be replaced by microorganisms derived from Rusitec. This technique involves the incubation of samples with buffered rumen fluid for 48 h followed by an extraction with a neutral-detergent solution.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2001 , 2001 , pp. 132
Copyright
Copyright © The British Society of Animal Science 2001

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References

Goering, M.K. and Van Soest, P.J.. 1970. Forage Fiber Analysis (apparatus, reagents, procedures and some applications). Agricultural Handbook No. 379, USDA, Washington DC.Google Scholar
Van Soest, P.J., Wine, R.H. and Moore, L.A.. 1966. Estimation of the true digestibility of forages by the in vitro digestion of cell walls. Proc. 10th Int. Grasslands Congr., Helsinki. Pp. 438–441.Google Scholar