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Comparison of procedures for estimating microbial contamination of feed residues in the in situ bag procedure of measuring protein degradation in the rumen of sheep

Published online by Cambridge University Press:  20 November 2017

F.K. Avornyo  and
E.L. Miller
F.K. Avornyo
Affiliation:
University of Cambridge, Department of Clinical Veterinary Medicine, 307 Huntingdon Road, Cambridge, CB3 OJQ, UK
E.L. Miller
Affiliation:
University of Cambridge, Department of Clinical Veterinary Medicine, 307 Huntingdon Road, Cambridge, CB3 OJQ, UK
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Extract

Feed residue following in situ incubation can be contaminated with rumen microbes, thereby underestimating feed protein degradation in the rumen. Three methods, namely labelling with 35S, measuring cytosine (C) as an internal microbial marker and dissolving the contaminating microbes in neutral detergent (ND) solution, were compared with the aim of choosing the method that best estimated microbial contamination.

The in situ protein degradations of grass hay and molassed sugarbeet pulp (mSBP) were studied. Incubation times were 0, 8, 16, 24, 48 and 72 h, with each incubation time replicated six times (3 sheep x 2 days). Each sheep received 1 kg of 60:40 hay:concentrate per day offered at two hourly intervals. Liquid- (LAM) and solid-associated microbes (SAM) were obtained according to the methods by Mathers & Aitchison (1981) and Whitehouse et al. (1994), respectively. Sulphur35 labelling was as described by Mathers & Aitchison (1981).

Type
Programme
Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 213
Copyright
Copyright © The British Society of Animal Science 1999

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References

Mathers, J.C. and Aitchison, E.M. 1981. Direct estimation of the extent of contamination of food residues by microbial matter after incubation within synthetic fibre bags in the rumen. Journal of Agricultural Science, Cambridge 96: 691.Google Scholar
Whitehouse, N.L., Olson, V.M., Schwab, C.G., Chesbro, W.R., Cunningham, K.D. and Lykos, T. 1994. Improved Techniques for Dissociating Particle-Associated Mixed Ruminal Micro-organisms from Ruminal Digesta Solids. Journal of Animal Science 72:1335–1343.Google Scholar