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Comparison of oven drying versus freeze drying on the analysis of non-starch polysaccharides in graminaceous and leguminous forages

Published online by Cambridge University Press:  20 November 2017

A.C. Longland
Affiliation:
Institute of Grassland and Environmental Research Plas Gogerddan, Aberystwyth, SY23 3EB
R.D. Pilgrim
Affiliation:
Institute of Grassland and Environmental Research Plas Gogerddan, Aberystwyth, SY23 3EB
I.H. Jones
Affiliation:
Institute of Grassland and Environmental Research Plas Gogerddan, Aberystwyth, SY23 3EB
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Extract

The carbohydrate portion of dietary fibre is increasingly being measured as non-starch polysaccharides (NSP). Unlike the more traditional, indirect gravimetric methods of measuring dietary fibre such as the crude fibre, acid detergent fibre, or neutral detergent fibre (NDF) techniques, the NSP method is a direct measurement of the monomeric constituents of the carbohydrate portion of the fibre fraction. Neutral NSP monomers are measured by gas chromatography of alditol acetate derivatives of acid hydrolysates of destarched samples, whereas the acidic sugars in the hydrolysates are determined by colorimetry (EnglystandCummings, 1984) or decarboxylation (Theander and Aman, 1979). As it has been shown that mode of drying can affect the values obtained for fibre determined gravimetrically by the NDF technique (Deinum and Maassen, 1994), it is important to know if NSP values are similarly affected. Therefore the effects of oven drying versus freeze drying on the NSP values obtained for various temperate forages was assessed.

Type
Techniques and Methods
Copyright
Copyright © The British Society of Animal Science 1995

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References

Deinum, B. and Maassen, A. (1994) Animal feed science and Technology, 46; 7586 10.1016/0377-8401(94)90066-3CrossRefGoogle Scholar
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Theander, O and Aman, P. (1979) Swedish Journal of Agricultural Research, 9:97106 Google Scholar