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Comparison of 15N and purines as microbial markers in a RUSITEC system

Published online by Cambridge University Press:  20 November 2017

M.D. Carro  and
E.L. Miller
M.D. Carro
Affiliation:
Dept. Producción Animal I, Universidad de León, 24071 León, Spain
E.L. Miller
Affiliation:
Dept. Clinical Veterinary Medicine, University of Cambridge, Cambridge CB3 OJQ, UK
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Extract

One of the problems of quantifying the microbial protein synthesis in the rumen is the lack of an ideal microbial marker. Purines and 15N are two of the most recommended microbial markers (Broderick and Merchen, 1992), but none of them has proven to be completely satisfactory. The aim of this study was to investigate the effect of using two different microbial markers (15N and purines) on the estimation of microbial production of four different diets in a RUSITEC system (Czerkawski and Breckenridge, 1977).

Two 14-day incubation trials were carried out with a RUSITEC system (8 vessels). In each vessel 12 g of fibre were incubated daily in nylon bags for 48 h. Half of the nitrogen (N) requirements was infused continuously as ammonia (NH4Cl). The other half of N was infused as protein, peptides, amino acids (AA) or ammonia. In each period, 2 vessels received each of the N treatments and a solution of (15NH4)2SO4 was infused as microbial marker.

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Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 214
Copyright
Copyright © The British Society of Animal Science 1999

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References

Broderick, G.A. and Merchen, N.R., 1992. Markers for quantifying microbial protein synthesis in the rumen. Journal of Dairy Science, 75: 2618–2632.Google Scholar
Carro, M.D. and Miller, E.L. 1998. Effect of supplementing a fibre basal diet with different nitrogen forms on ruminal fermentation and microbial growth in RUSITEC system. British Journal of Nutrition, (Submitted).CrossRefGoogle Scholar
Czerkawski, J.W. and Breckenridge, G., 1977. Design and development of a long-term rumen simulation technique (Rusitec). British Journal of Nutrition, 38: 371–384.CrossRefGoogle ScholarPubMed