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Cellulase and xylanase activity of the rumen anaerobic fungi grown on untreated and sodium hydroxide treated barley straw

Published online by Cambridge University Press:  20 November 2017

M. Rezaeian ,
D. S. Parker  and
G. W. Beakes
M. Rezaeian
Affiliation:
Department of Animal Breeding & Nutrition, University of Tehran, Tehran, Iran
D. S. Parker
Affiliation:
Department of Biological & Nutritional Science, University of Newcastle Upon Tyne, NE1 7RU
G. W. Beakes
Affiliation:
Department of Biological & Nutritional Science, University of Newcastle Upon Tyne, NE1 7RU
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Extract

The treatment of straw with sodium hydroxide in order to upgrade its nutritive value and to increase the utilisation of its energy by ruminants has been widely employed in many parts of the world. The aim of the experiment was to assess the fibrolytic activity of the anaerobic fungi in vitro using either untreated or NaOH treated barley straw.

Anaerobic fungi were isolated from the rumen of a sheep fed with a diet of hay and lucerne pellets using medium C based on that described by Davies et al. (1993) and as modified by Rezaeian (1996). The Isolates were inoculated into two series of culture media containing either sodium hydroxide treated (TS) or untreated milled straw (US). Five replicate cultures were prepared and incubated at 39 °C over a period of 12 days. The dry matter loss of the substrates and changes in pH of the medium cultures were measured.

Type
Programme
Information
Proceedings of the British Society of Animal Science , Volume 1999 , 1999 , pp. 152
Copyright
Copyright © The British Society of Animal Science 1999

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References

Davies, D., Theodorou, M. K., Lawrence, M. I. G., and Trinci, A. P. J. 1993. Distribution of anaerobic fungi in the digestive tract of cattle and their survial in faeces. Journal of General Microbiology 139: 1395–1400.Google Scholar
Miller, G. L., Blum, R., Glennon, W. E. and Burton, A. L. 1960. Measurement of carboxymethylcellulase activity. Analytical Biochemistry 2: 127–132 Google Scholar
Rezaeian, M. 1996. Assessment and distribution of anaerobic fungi in the ruminant gut. Ph.D. Thesis, University of Newcastle Upon Tyne Google Scholar