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Assessing scrapie risk in dairy sheep flocks

Published online by Cambridge University Press:  20 November 2017

A. Psifidi*
Affiliation:
Aristotle University, Thessaloniki, Greece
C. I. Dovas
Affiliation:
Aristotle University, Thessaloniki, Greece
Z. Basdagianni
Affiliation:
Breeder’s cooperative Macedonia, Thessaloniki, Greece
I. Buzalas
Affiliation:
Aristotle University, Thessaloniki, Greece
G. Banos
Affiliation:
Aristotle University, Thessaloniki, Greece
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Extract

Scrapie is a degenerative disorder of the central nervous system of small ruminants. Scrapie in sheep appears to be controlled by genetic factors, with polymorphisms at codons 136, 154 and 171 of the PrP gene locus being the determining parameters (Hunter et al., 2005). Individual PrP genotypes are detected primarily with blood or ear notch tissue samples. Increasingly strict regulations on food safety and public hygiene require new, practical, animal friendly methods for large scale implementation. The first aim of this study was to develop a novel genotyping method using DNA extracted from milk somatic cells from individual animal samples. The second aim was to develop a quick, easy and accurate method for assessing the prevalence of undesirable genotypes within each flock using bulk milk.

Type
Theatre Presentations
Copyright
Copyright © The British Society of Animal Science 2008

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References

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