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Application of DNA amplification for genotyping cattle from milk

Published online by Cambridge University Press:  20 November 2017

K. Derecka ,
M. Hunter ,
M.D. Royal ,
S. Watters  and
A.P.F. Flint
K. Derecka*
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, Leics LE12 5RD, U.K.
M. Hunter
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, Leics LE12 5RD, U.K.
M.D. Royal
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, Leics LE12 5RD, U.K.
S. Watters
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, Leics LE12 5RD, U.K.
A.P.F. Flint
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington, Loughborough, Leics LE12 5RD, U.K.
*
Email: Kamila.Derecka@Nottingham.ac.uk
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Extract

Blood is a good source of DNA, although other tissues such as skin biopsies or hair follicles can be used (Heal et al, 2002). Milk, which is widely available and obtained non-invasively, would be the source of choice in lactating animals. However, it is difficult to extract DNA from small samples of milk in quantities sufficient for simultaneous genotyping at multiple loci. The objective of this study was to determine whether genomic DNA isolated from small amounts of milk could be amplified to provide sufficient material for running multiple genotyping reactions.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2004 , 2004 , pp. 221
Copyright
Copyright © The British Society of Animal Science 2004

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References

Aasen, E., Medrano, J.F. 1990. Amplification of the ZFY and ZFX genes for sex identification in humans, cattle, sheep and goats. Biotechnology 8: 1279–81.Google ScholarPubMed
Heal, P.J., Dennis, J.A., Windsor, P.A., Pierce, K.D., Schofield, P.A. 2002. Genotyping cattle for inherited congenital myoclonus and maple syrup urine disease Austrian Veterinary Journal 80: 695–7.CrossRefGoogle Scholar
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