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Preliminary observations on skatole production in pigs

Published online by Cambridge University Press:  22 November 2017

M. Hawe ,
N. Walker ,
M. Porter  and
B.W. Moss
M. Hawe
Affiliation:
Agricultural Research Institute of N Ireland, Hillsborough, Co Down, BT26 6DR
N. Walker
Affiliation:
Agricultural Research Institute of N Ireland, Hillsborough, Co Down, BT26 6DR
M. Porter
Affiliation:
Agricultural Research Institute of N Ireland, Hillsborough, Co Down, BT26 6DR
B.W. Moss
Affiliation:
Agriculture and Food Science Centre, Newforge Lane, Belfast BT9 5PX
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Extract

Skatole and indole, compounds formed in the large intestine by microbial breakdown of tryptophan, are associated with carcass taint (Hansson et al., 1980). The present work involved the development of a method for detecting the absolute values of the analytes in fat and faeces. The effect on faecal excretion of skatole was also examined following infusion of skatole directly into the caecum. Finally as substrate effects the type and extent of fermentation in the large intestine, different diets were fed and the corresponding faecal and fat skatole levels were determined.

Skatole analysis involved homogenising adipose tissue with methanol and removal of fat by cooling and filtering. Solid phase extraction columns concentrated the analytes to produce samples which gave good resolution by gas liquid chromatography (Porter, Hawe and Walker, 1989). Due to higher levels of skatole in faeces, no concentrating phase was required before detection.

Type
Pig and Poultry Production
Information
Proceedings of the British Society of Animal Production (1972) , Volume 1989 , March 1989 , pp. 81
Copyright
Copyright © British Society of Animal Production 1989

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References

Hansson, K.E., Lundstrom, K., Fjelkner-Modig, S., and Persson, J., 1980. The importance of androstenone and skatole for boar taint. Swedish Journal of Agricultural Research 10: 167–173.Google Scholar
Porter, M., Hawe, M and Walker, N., , 1989. Journal of Science Food and Agriculture. In press.Google Scholar