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In Vitro Fertilization of Bovine Oocytes Matured In Vitro

Published online by Cambridge University Press:  21 November 2017

K.H. Lu
Affiliation:
Department of Agriculture, University College, Dublin
I. Gordon
Affiliation:
Department of Agriculture, University College, Dublin
M.P. Boland
Affiliation:
Department of Agriculture, University College, Dublin
T.F. Crosby
Affiliation:
Department of Agriculture, University College, Dublin
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Extract

The development of an efficient laboratory procedure which would enable cattle ovarian oocytes to be matured in vitro, fertilized and cultured in vitro to the blastocyst stage of development could have important practical and scientific implications. The commercial exploitation of certain embryo transfer techniques applicable in cattle (eg., twinning by embryo transfer) might be facilitated by the development of such a procedure and there would be many advantages to having a cheap source of embryos available for research purposes. The present report deals with some of the studies recently carried out in this laboratory aimed at utilising follicular oocytes recovered from the ovaries of cattle slaughtered for beef at the abattoir. Such studies have been undertaken over a period of almost twenty years, starting with the work of Sreenan (1968)* but it now realised that the oocytes of farm mammals are incapable of normal development until after the completion of complex changes during maturation.

Type
Reproduction
Copyright
Copyright © The British Society of Animal Production 1997

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