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Variation in pre-harvest sprouting resistance, seed germination and changes in abscisic acid levels during grain development in diverse rice genetic resources

Published online by Cambridge University Press:  12 October 2016

Gi-An Lee ,
Young-Ah Jeon ,
Ho-Sun Lee ,
Do-Yun Hyun ,
Jung-Ro Lee ,
Myung-Chul Lee ,
Sok-young Lee ,
Kyung-Ho Ma  and
Hee-Jong Koh
Gi-An Lee
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, 370 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54874, Republic of Korea Crop Science and Biotechnology, Seoul National University, 599 Gwanak-Ro, Gwanak-Gu, Seoul-Si 08826, Republic of Korea
Young-Ah Jeon
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, 370 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54874, Republic of Korea
Ho-Sun Lee
Affiliation:
International Technology Cooperation Center, RDA, 300 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54875, Republic of Korea
Do-Yun Hyun
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, 370 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54874, Republic of Korea
Jung-Ro Lee
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, 370 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54874, Republic of Korea
Myung-Chul Lee
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, 370 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54874, Republic of Korea
Sok-young Lee
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, 370 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54874, Republic of Korea
Kyung-Ho Ma
Affiliation:
National Agrobiodiversity Center, National Institute of Agricultural Science, RDA, 370 Nonsaengmyeong-Ro, Wansan-Gu, Jeonju-Si, Jeollabuk-Do 54874, Republic of Korea
Hee-Jong Koh*
Affiliation:
Crop Science and Biotechnology, Seoul National University, 599 Gwanak-Ro, Gwanak-Gu, Seoul-Si 08826, Republic of Korea
*
*Corresponding author. E-mail: heejkoh@snu.ac.kr
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Abstract

Among domesticated traits, pre-harvest sprouting (PHS) caused by the early breakage of dormancy leads to severe economic losses. Therefore, regulating PHS is important for cereal crop improvement against changes in climate. In this study, we surveyed naturally occurring variations in seed germination in diverse rice germplasm for the available resources of this trait, and investigated the changes of abscisic acid (ABA) levels during grain development by the distinguished PHS-resistant groups. We discovered wide variations in germination among the 205 rice accessions examined and found that 90 accessions are resistant (germination <20%) to PHS. Tropical and subtropical accessions, which are subjected to long wet periods, are more resistant to PHS than the other accessions. We detected an increase in germination of detached seeds from the panicle compared with intact seeds in panicle at harvesting time. This might be attributed to a weakening of the mechanical barrier that prevents water imbibition and radical emergence. ABA levels were maximal at 10 d after flowering and decreased thereafter. Interestingly, PHS-susceptible accessions maintained higher or similar ABA levels compared with PHS-resistant accessions, suggesting that the key factors for seed dormancy and its breakage are ABA perception and signal transduction rather than total ABA content. The diversity of germination ability detected in this study could be sustainably used for crop improvement and to help unveil the genetic and physiological basis of this quantitative trait.

Keywords

ABAdormancygenetic resourcesgermination abilityrice
Type
Research Article
Information
Plant Genetic Resources , Volume 16 , Issue 1 , February 2018 , pp. 18 - 27
Copyright
Copyright © NIAB 2016 

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